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pLenti-EF1-MCS-PGK-GFP Lentiviral Expression Vector


Lentivirus vector based on the human immunodeficiency virus-1 (HIV-1) has become a promising vector for gene transfer studies. The advantageous feature of lentivirus vector is the ability of gene transfer and integration into dividing and nondividing cells. The pseudotyped envelope with vesicular stomatitis virus envelope G (VSV-G) protein broadens the target cell range. Lentiviral vectors have been shown to deliver genes to neurons, lymphocytes and macrophages, cell types that previous retrovirus vectors could not be used. Lentiviral vectors have also proven to be effective in transducing brain, liver, muscle, and retina in vivo without toxicity or immune responses. Recently, the lentivirus system is widely used to integrate siRNA efficiently in a wide variety of cell lines and primary cells both in vitro and in vivo. Lentivirus particles are produced from 293T cells through transient transfection of plasmids that encode for the components of the virion. Our third generation lentiviral systems have been designed for increased researcher safety. pLenti-EF1-MCS-PGK-GFP Lentiviral Expression Vector contains the multiple cloning sites (MCS) downstream of EF1α promoter and copGFP reporter driven by PGK promoter. The multiple restriction enzyme sites are included for convenient cloning of the gene of interest.

Note: Bacterial culture of pLenti vectors should be done in medium containing 10 μg/mL Ampicillin. For maximal plasmid yield and quality, we recommend Stbl3 competent cells (Invitrogen).
Product Name pLenti-EF1-MCS-PGK-GFP Lentiviral Expression Vector
Shipping Condition

Room Temperature - 2 Day Shipping

Storage and Stability

Store at -20°C immediately upon receipt. This product is stable for 6 months when stored as directed.

Quality Control

This plasmid is sequence verified.

Restricted Use

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Documents Product Specification Sheet LV210 pLenti-EF1-MCS-PGK-GFP.pdf