Also available at      &   VWR
1-Step 2X RT-PCR Master Mix-TaqMan ProbeMake RNA analysis as easy as DNA analysis.

Name

1-Step 2X RT-PCR Master Mix-TaqMan Probe

Buy from

Application

Make RNA analysis as easy as DNA analysis.
- The 1-Step 2X RT-PCR Master Mix-TaqMan probe is used for real-time qualitative and quantitative RT-PCR with TaqMan probe.
- The master mix is a premixed, 2X concentrated solution that has all the components except for gene-specific primers and RNA template.

Features

- For the reverse transcription step, this kit uses a highly efficient thermophilic Reverse Transcriptase (US patent pending), which is a thermophilic type A polymerase with optimal temperatures of 60-62°C, and it is heat-inactivated at ≥90°C.
- The RTase efficiently synthesizes a complementary DNA strand on RNA template, ≤1 unit per 20 μL of reaction.
- The RTase can detect single digit copies of target RNA molecules.
- This kit also contains Taq-Probe DNA polymerase for the amplification step, specially engineered for TaqMan probes and generating S-Shaped curves.
- This kit is designed for singleplex RT-PCR.
- The preferable PCR product size is ≤150bp.
Mouse Tail DNA Extraction only 20 minutes
3D Cell Culture Gel 30% < Mkt Price
PCR Kits 50% < Mkt price
Beta-Hexosaminidase Activity Colorimetric Assay Fast and sensitive, High-throughput
Endotoxin-Free Plasmid Kits maxi, midi and mini-prep