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Plant Tissue Total Protein Extraction Kit


8-min. Plant Tissue Total Protein Extraction Kit

Cat. #

(50 reactions)

Researchers also buy our Protein Analysis Reagents (HRP, Antibody enhancer, WB stripping solution, etc.)
Also can buy from :


Extract total protein from plant tissues within 8 minutes.

Can handle frozen or fresh leaves, seeds, soft stem and roots, etc.

Extract native or denatured protein.

For SDS-PAGE, immunoblottings, ELISA, IP, enzyme assays etc.

Extracted proteins can be use as a good starting material for small scale protein purification in column chromatography.

This product is for research use only.

Product Size

P508L: 50 rxn


This kit is for fast extraction of total proteins from plant tissues.

  • Fast - 5 to 8 minutes
  • High yield and high concentration - 2 ~ 8 mg/ml
  • Ideal for small volume of sample - can handle 20 ~ 200 mg plant tissue.

Shipping / Storage

Ship at room temperature. Store "Native lysis buffer" at 4℃, and the rest at room temperature. (Do not freeze.)

Shelf Life

12 months

Manual (protocol)
  8-min. Plant tissue Total Protein Extraction Kit


Citation 1:
Xianjun Peng, Qi Wang, Hui Liu,Shihua Shen. Phylogenetic and functional analysis of the basic transcription factor gene BTF3 from Jatropha curcas. Plant Growth Regul. June 2017, Volume 82, Issue 2, pp 247–257.


Components Amount Storage
Denaturing lysis buffer 25 mL Room temperature
Native lysis buffer 25 mL Room temperature
Filter Cartridges 50 Room temperature
Collection tubes with cap 50 Room temperature
Plastic rods 4 Room temperature


  • Protein profiles extracted by denaturing and native lysis buffer are not identical. For a given application one lysis buffer might be superior to the other. This kit provides both denaturing and native cell lysis buffers for users to test and select the best one for a specific application.
  • Total proteins can be extracted from plant tissue in 5-8 min
  • Protease inhibitors is not necessary prior to extraction. However if downstream application takes significant amounts of time or the protein extract will be stored for longer period of time, addition of protease inhibitors to lysis buffer is recommended.
  • For determination of protein concentration, BCA kit (Pierce) is recommended.
  • To study protein phosphorylation, phosphatase inhibitors (such as PhosStop from Roche) should be added to lysis buffer prior to use.
  • This protocol is developed and validated by 101Bio’s OEM partner. Spin column based protein extraction and cell fractionation technologies were developed by 101Bio’s OEM partner.
Plant Tissue Total Protein Extraction Kit
Protein Analysis Reagents
Name Cat. # Size Price Order
101Bio HRP Substrate Kit P5W1 30 ml /30 ml $  99.00
101Bio Antibody Enhancer P5W2 30 ml $  129.00
101Bio WB Stripping Solution P5W3 250 ml $  129.00
101Bio Ponceau S Stain P5W4 250 ml $  59.00
101Bio Peroxidase Suppressor P5W5 30 ml $  89.00
101Bio High Efficiency Protein Precipitation kit P5W6 30 ml /30 ml $129.00
101Bio Red Blood Cell Lysis Buffer P5W7 250 ml $  109.00
101Bio WB Blocking Solution P5W8 250 ml $  109.00
101Bio Denaturing Protein Solubilization Reagent P5W9 10 ml $  49.00
101Bio Non-Denatured Protein Solubilization Reagent P5W10 10 ml $  49.00
101Bio Protein Solubilization Reagent for MS P5W11 2 ml $  49.00
101Bio SDS-Remover P5W12 10 ml $  79.00
101Bio Albumin Depletion Reagent for Plasma and Serum P5W13 10 ml $  109.00
101Bio Protein A+G Agarose Beads W0349 1 ml $  99.00
101Bio Protease Inhibitor Cocktail (100x) W2200 1 ml $  49.00
101Bio Yeast Total Protein Extraction Kit for SDS-PAGE Y203-50 50 rxn $  80.00
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