Name

1 kb DNA Ladder 2.0 (1 kb ~ 10 kb, with loading dye)

Cat. #

Application

1 kb DNA Ladder 2.0 is a mixture of 8 fragments: 1 kb, 2 kb, 3 kb, 4 kb, 5 kb, 6 kb, 8 kb, 10 kb. The 5 kb band is brighter than other bands as internal reference indicators. This product contains 1x Loading Buffer and can be directly load to gel. When loading 5 µL, the 5 kb band contains 150 ng DNA, and other bands contains 50 ng DNA. Concentration: 500 ng / 5 μL

Load 5 µL of 1 kb DNA Ladder 2.0 on agarose gel to the well of agarose gel. If the well is wider than 6mm, increase the sample volume proportionally (1 µL / mm). Run electrophoresis.

Shipping / Storage

Manual (protocol)

Result

The gel image shown is 5 µL of 1 kb DNA Ladder 2.0 separated by 0.7% agarose gel.

• Do not heat before loading.
• Please use fresh electrophoresis buffer and agarose gel for the best result.
• High quality agarose should be used to avoid effecting the DNA bands and the recommended agarose gel concentration is 0.7% ~ 1%, recommended voltage is 4 ~ 8 v / cm.
• The separation performance of DNA fragments in agarose gel is related to gel concentration. The higher concentration gel separates shorter DNA fragments better. The lower concentration gel separates longer DNA segments better.
• This product is not for diagnostic use. The safety and efficacy of this product in diagnostics or other clinical uses have not been established.