ExoFectin® Plasmid DNA-into-Exosome Kit (DNA transfection kit for exosome) is for transfecting plasmid DNA into exosomes isolated by our PureExo® kits. This product is for research use only.
Product Size
100 µl ExoFectin Reagent (~ 20 reactions)
Yield
50% ~ 90% transfection efficiency
Description
ExoFectin® Plasmid DNA-into-Exosome Kit (DNA transfection kit for exosome)
is a unique blend of polymers designed for the delivery of plasmid DNA into exosomes. This transfection reagent provides highly efficient transfection of plasmid DNA into exosomes, allowing modified exosomes to carry and deliver plasmid DNA into target cells. This kit provides the following advantages:
efficient gene delivery
gentle on treated exosomes
no DNA aggregation in exosome
Shipping / Storage
Ship and store at 4℃. (Do not freeze.)
Shelf Life
6 months
Manual (protocol)
Components
Components
Amount
Storage
ExoFectin Reagent
100 μl
4℃
Supplemental buffer
1000 μl
4℃
Positive control plasmid DNA (green fluorescence, 0.5µg/µl)
10 μl
4℃
Remarks
The efficiency of transfection depends on the quality of the exosomes. We do not suggest to start with impure exosome samples prepared by PEG method.
The efficiency of transfection also depends on the concentration of the exosomes and DNA. For best result, we suggest customers to test the optimal DNA / exosome concentration for their own experiment.