Answer: By targeted filtration, our filter of specific binding treatment removes protein contaminant to isolate exosome at 95% high purity.
Click to see pure exosome images
Isolate pure exosome from stem cell culture media (supernatant). This kit yields much higher purity than exosome precipitation method. This product is for research use only.
Yield
Each reaction can process 20 mL stem cell culture medium. The yield of each reaction can yield pure exosome suspended in 50 ~ 200 µL PBS .
Description
P107 kit is for exosome purification / isolation at high yield and purity from stem cell culture media.
Easy to use: No ultra-centrifugation (< 2 hours)
10 fold higher yield (vs. other kits and ultracentrifuge)
Save cost (vs. antibodies-beads method)
Isolate Pure exosome (>95% purity vs. 25% purity by exosome precipitation method)
Intact exosome (good morphology)
This kit isolate high yield pure exosomes for any downstream applications: EM study, exosome label, exosome subpopulation, qRT-PCR profiling of exosomal miRNAs, and gel analysis of exosomal proteins.
Shipping / Storage
Ship and store at room temperature. Upon receiving keep all bottles upright, in cool and dark place.
Shelf Life
12 months
Manual (protocol)
101Bio.com Exosome Isolation Kit
Data Sheet
Components
Components
Amount
Storage
Cat.#: P107S
Cat.#: P107
Solution A (blue)
1.5 mL
7.5 mL
Room temperature
Solution B
1.5 mL
7.5 mL
Room temperature
Solution C
6 mL
10 mL x 3 bottles
Room temperature
PureExo® Column
2
10
Room temperature
Remarks
The maximum medium volume of each reaction is 20 mL from at most 1 x 107 cells. Do not exceed the suggested sample volume or the cell number. Otherwise it may cause indistinct layer separation and column clogging. One PureExo® Column is only for one reaction.