Real in vivo grade, recombinant antibodies, endotoxin < 1 EU/mg
Mouse IgG2b Isotype Control Antibody
Recombinant Mouse IgG2b Reference Antibody
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Description
SA130: In vivo Grade Recombinant Mouse IgG2b Isotype Control Antibody (mIgG2b Isotype Control)
The in vivo grade recombinant mouse IgG2b isotype control antibody (mIgG2b isotype control) was produced in HEK 293 cells.
Immunogen: N/A.
Clone: 12B9.
Isotype: mouse IgG2b, kappa.
Applications: an isotype-matched negative control for mouse IgG2b kappa antibodies used in ELISA, Western Blot (WB), Flow Cytometry (Flow), Immunoprecipitation (IP), Immunohistochemistry (Paraffin) (IHC (P)), Immunohistochemistry (Frozen) (IHC (F)), and in vivo animal model research.
Formulation: 0.2 μM filtered solution of 1x PBS.
Purity: >95% by SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.
Shipping: The product is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
1 month from date of receipt, 2 to 8°C as supplied.
3 months from date of receipt, -20°C to -70°C as supplied.
Background
Mouse IgG antibodies are classified to four subclasses mainly based on the isotype of the heavy chain (IgG1, IgG2a or IgG2c, IgG2b, and IgG3 for the heavy chain; kappa and lamda for the light chain), of which IgG1 and kappa are the most abundant named in serum. IgG antibodies are large molecules of about 150 kDa composed of four peptide chains, two identical heavy chains of about 50 kDa and two identical light chains of about 25 kDa. The four peptides are arranged in a Y-shape tetramer by disulfide bonds formed between the two heavy chains and between a heavy chain and a light chain.
Mouse Tail DNA Extraction | only 20 minutes | |
3D Cell Culture Gel | 30% < Mkt Price | |
PCR Kits | 50% < Mkt price | |
Beta-Hexosaminidase Activity Colorimetric Assay | Fast and sensitive, High-throughput | |
Endotoxin-Free Plasmid Kits | maxi, midi and mini-prep |