Also available at    &     VWR
Hipro Circulating Cell-Free DNA (cfDNA) Isolation Kit
from human plasma, serum, urine and other body fluid

Name

Hipro Circulating Cell-Free DNA (cfDNA) Isolation Kit
from human plasma, serum, urine and other body fluid

Cat. #

W2603-10
$109.00  
10 reactions
W2603-20
$169.00  
20 reactions
W2603-50
$299.00  
50 reactions

Application

Sample Yield Elution Method DNA Quality
0.1 ~ 1 mL body fluid up to 5 μg circulating DNA 20 ~ 100 μL Spin Column PCR, qPCR, Next-Gen Sequencing
This kit with special spin column is used for small amount of cfDNA isolation. The recovery rate is higher than other kits or methods.
Protocol time: 1 hour
This product is for research use only.

Description and features

This kit enables efficient isolation of circulating 50 ~ 15,000 bp cell-free DNA (cfDNA) from fresh or frozen cell-free body fluid such as serum, plasma, lymph fluid or urine.

This kit's special spin column can bind small amount of cell free circulating DNA while contaminants pass through. PCR inhibitors (salts, proteins and lipids) are removed in wash steps. Pure DNA is then eluted by elution buffer provided in the kit. One spin column can process 0.1 ~ 1 mL body fluid sample.

The purification is highly reliable and reproducible. The purified DNA can be used for PCR and quantitative real-time PCR and RT-PCR, etc.

serum circulating cell-free DNA (cfDNA) isolation kit
101Bio's Circulating Cell-Free DNA (cfDNA) Isolation Kit yield much higher than competitor's kit.
serum circulating cell-free DNA (cfDNA) isolation kit
101Bio's Circulating Cell-Free DNA (cfDNA) Isolation Kit yield much higher than competitors' kits.
serum circulating cell-free DNA (cfDNA) isolation kit
The result of circulating cell-free DNA extraction by 101Bio's kit is highly consistent and reproducible.

Shipping / Storage

Ship and store at room temperature. After reconstitution, store Proteinase K at -20oC

Shelf Life

12 months

Manual (protocol)

  101Bio.com
  Hipro Circulating Cell-Free DNA (cfDNA) Isolation Kit

Components

Components Amount Storage
W2603-20 W2603-50
Buffer CL 20 mL 45 mL Room temperature
Buffer CB (concentrate) 24 mL 60 mL Room temperature
Buffer GW1 (concentrate) 6 mL 13 mL Room temperature
Buffer GW2 (concentrate) 6 mL 15 mL Room temperature
Buffer EB 6 mL 15 mL Room temperature
Proteinase K * 50 mg 100 mg Room temperature /
-20oC after reconstitution
Proteinase K Storage Buffer 2.5 mL 5 mL Room temperature
Spin Column MF 20 50 Room temperature
Collection Tube (2 mL) 20 50 Room temperature
Collection Tube (1.5 mL) 20 50 Room temperature
* Store in -20oC after reconstitution. Avoid sitting at room temperature for long time, and avoid repeated freeze-thaw cycles.

Protocol Diagram

serum circulating cell-free DNA (cfDNA) isolation kit
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