Also available at    &     VWR
Endotoxin-Free Plasmid Maxiprep Kit

Name

Endotoxin-Free Plasmid Maxiprep Kit

Cat. #

W2104-10
$89.00
(10 rnx)            order from  Fisher (Fisher Cat.# NC1492023)
Also can buy from :

Application

Sample Yield Elution Method DNA Quality
150~300mL E. Coli 200μg~1.5mg EndoFree plasmid DNA 1 ~ 3 mL Spin Column Transfection, PCR, Enzyme digestion, Sequencing
This product is for research use only.

Description and features

Eukaryotic cells are very sensitive to endotoxin, a common contaminant in the plasmid extraction, and the transfection efficiency of the cells will greatly reduce if the plasmid contains endotoxin. This kit can efficiently extract endotoxin-free plasmid in a fast and simple way. The endotoxin, genomic DNA, RNA, protein and other impurities can be largely removed.

200 μg ~ 1.5 mg transfection-grade plasmid DNA can be purified from 150 ~ 300 mL overnight culture of E. Coli in LB medium with this kit within 50 minutes.

The extracted DNA can be directly used for Transfection PCR, restriction enzyme digestion, sequencing, etc. No need to precipitate, concentrate or desalt.

Shipping / Storage

Ship at room temperature. Store RNase A or RNase A added buffer P1 at 4oC

Shelf Life

12 months

Manual (protocol)

  101Bio.com
  Endotoxin-Free Plasmid Maxiprep Kit

General Procedure

Endotoxin-Free Plasmid DNA Maxiprep Kit, EndoFree Plasmid DNA purification Kit

Components

Components Amount Storage
Buffer P1 125 mL Room temperature; store at 4oC if RNase A is added.
Buffer P2 125 mL Room temperature
Buffer E3 125 mL Room temperature
Buffer PS 30 mL Room temperature
Buffer PW ( concentrate ) 50 mL Room temperature
Endotoxin-Free Buffer EB 30 mL Room temperature
RNase A ( 10 mg/mL ) 2 mL Room temperature
Plunger 10 Room temperature
Endotoxin-Remover Filter 10 Room temperature
Spin Column DQ with collection tube 10 Room temperature
Collection Tube 10 Room temperature
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