1-Step 2X Fast RT-PCR Master Mix-SYBR GreenMake RNA analysis as easy as DNA analysis.
Name |
1-Step 2X Fast RT-PCR Master Mix-SYBR Green |
||||||||||||||||
|
|||||||||||||||||
Application |
Make RNA analysis as easy as DNA analysis.
- The 1-Step 2X Fast RT-PCR Master Mix is used for qualitative and quantitative RT-PCR amplifications with SYBR Green dye for fast thermocycling. - This master mix is a premixed, 2X concentrated solution that has all the components except for gene-specific primers and RNA template. |
||||||||||||||||
Features |
- For the reverse transcription step, this kit uses a highly efficient thermophilic Reverse Transcriptase (US patent pending), which is a thermophilic type A polymerase with optimal temperatures of 60-62°C, and it is heat-inactivated at ≥90°C.
- The RTase efficiently synthesizes a complementary DNA strand on RNA template from a gene-specific primer, ≤1 unit per 20μL of reaction. - The RTase can detect single digit copies of target RNA molecules. - The kit also contains Taq-Fast DNA polymerase for the amplification step, which extends more than 300 bases with short cycling program. - The kit is designed for singleplex RT-PCR. - The preferable PCR product size is ≤150bp |
||||||||||||||||
Manual (protocol) |
 |
||||||||||||||||
Q&A |
|
||||||||||||||||
 |
Mouse Tail DNA Extraction | only 20 minutes |
|
3D Cell Culture Gel | 30% < Mkt Price |
|
PCR Kits | 50% < Mkt price |
|
Beta-Hexosaminidase Activity Colorimetric Assay | Fast and sensitive, High-throughput |
|
Endotoxin-Free Plasmid Kits | maxi, midi and mini-prep |