1-Step 2X Multiplex RT-PCR Master Mix-TaqMan ProbeMake RNA analysis as easy as DNA analysis.
Name |
1-Step 2X Multiplex RT-PCR Master Mix-TaqMan Probe |
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Application |
Make RNA analysis as easy as DNA analysis.
- The 1-Step 2X Multiplex RT-PCR Master Mix is used for qualitative and quantitative multiplex RT-PCR amplifications with TaqMan probes for up to 4 targets. - The master mix is a premixed, 2X concentrated solution that has all the components except for gene-specific primers, probes and RNA templates. |
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Features |
- For the reverse transcription step, this kit uses a highly efficient Thermophilic Reverse Transcriptase (US patent pending), which is a thermophilic type A polymerase with optimal temperatures of 60-62°C, and it is heat-inactivated at ≥90°C.
- The RTase efficiently synthesizes complementary DNA strands on RNA templates from gene-specific primers, ≥1 unit per 20μL of multiplex reaction. - The RTase can detect single digit copies of target RNA molecules. - This kit also contains Taq-Probe DNA polymerase for the amplification step, specially engineered for TaqMan probes and generating S-Shaped curves. - Two–four pairs of gene-specific primers can be applied in one reaction. - The preferable PCR product sizes are ≤150bp. |
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Manual (protocol) |
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Q&A |
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Comparison |
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