1-Step 2X RT-PCR Master Mix-SYBR Green DyeMake RNA analysis as easy as DNA analysis.
Name |
1-Step 2X RT-PCR Master Mix-SYBR Green Dye |
||||||||||||||||
|
|||||||||||||||||
Application |
- The 1-Step Fast 2X RT-PCR Master Mix is used for real-time qualitative and quantitative RT-PCR amplifications with SYBR Green dye particularly for fast thermocycling.
- The master mix is a premixed, 2X concentrated solution that has all the components except for gene- specific primers and RNA template. Research use only. |
||||||||||||||||
Features |
- The kit is designed for singleplex RT-PCR with SYBR Green dye.
- For the reverse transcription step, this kit uses a highly efficient Thermophilic Reverse Transcriptase (US patent pending), which is a thermophilic type A polymerase, with optimal temperatures of 60-62°C. - The RTase is easily heat-inactivated at ≥90°C for 1min. - The RTase efficiently synthesizes a complementary DNA strand on RNA template from a gene-specific primer, ≤1 unit per 20μL of reaction. - The RTase reversely-transcribes single digit copies of target RNA molecules consistently. - The kit also contains Taq DNA polymerase for PCR with SYBR Green dye. - The concentrations of the primers are variable depending on assay designs and thermocycling protocols (Table 1). - The preferred PCR product size is ≤150bp. - The kit has three formulations of ROX, Low ROX or High ROX concentrations for your choice. |
||||||||||||||||
Manual (protocol) |
 |
||||||||||||||||
Q&A |
|
||||||||||||||||
 |
Mouse Tail DNA Extraction | only 20 minutes |
|
3D Cell Culture Gel | 30% < Mkt Price |
|
PCR Kits | 50% < Mkt price |
|
Beta-Hexosaminidase Activity Colorimetric Assay | Fast and sensitive, High-throughput |
|
Endotoxin-Free Plasmid Kits | maxi, midi and mini-prep |