Thermophilic Reverse TranscriptaseMake RNA analysis as easy as DNA analysis.
Name |
Thermophilic Reverse Transcriptase |
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Application |
Make RNA analysis as easy as DNA analysis.This product is used for real-time fluorescent RT-PCR with either SYBR Green dye or TaqMan probe. | ||||||||||||||||
Features |
- The reverse transcriptase is a thermophilic type A polymerase (US patent pending) with optimal temperatures at 60-62°C, and it is heat-inactivated at ≥90°C (User Manual Tables 1-2).
- The enzyme efficiently synthesizes a complementary DNA strand on RNA template from a gene-specific primer, ≤1 unit per 20μL of reaction. - The enzyme can detect single digit copies of target RNA. - The preferable PCR product size is ≤150bp. - Designed for one-step real-time qualitative and quantitative RT-PCR. |
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Manual (protocol) |
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Q&A |
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