Name

2x Es Taq Master Mix (with dye)

Cat. #

Application

• Routine PCR
• T/A cloning

Specifications

This Master Mix contains Es Taq DNA Polymerase, PCR buffer, Mg2+, dNTP, PCR stabilizer and PCR enhancer. The concentration is 2x.

Es Taq DNA Polymerase possesses 5´→3´ DNA polymerase and 5´→3´ exonuclease activity. The polymerase has the high amplification efficiency and low mismatching as Taq DNA polymerase and high fidelity of Pfu DNA polymerase. Es Taq Polymerase catalyzes the non-template directed addition of an adenine residue to the 3´-end of both strands of DNA molecules to make it suitable for T/A cloning. The amplification range of Es Taq is ~ 6 kb.

This unique Master Mix recipe makes the system very reliable. More than 98% of PCR reaction can get successful amplification during the first try. It also works well on complicate templates.

The Master Mix contains dye, and can directly run electrophoresis after PCR reaction.

Shipping / Storage

Quality control

Manual (protocol)

PCR reaction system

Note: The recommended primer concentration for PCR is between 0.1-1.0 µM of each primer. The use of higher concentrations of primers can have higher amplification effect. Low primer concentration will generally ensure cleaner product and lower background.

PCR reaction conditions

Note:

• The recommended annealing temperature is about 5℃ below Tm of primers. If extra bands are observed, higher annealing temperatures should be considered. The absence of product indicates the need for a lower annealing temperature.
• PCR extension time is depended on the size of target gene sequence. Es Taq DNA polymerase is approximately 1 kb DNA / 30 seconds.
• The number of PCR cycles will basically depend on the downstream application of the PCR product.

This Master Mix contains dye for electrophoresis. After PCR, directly load 5 µL of PCR product to agarose gel to run electrophoresis. No need to add loading buffer.