Name

2x GoldStar Best PCR Master Mix (with dye)

Cat. #

Application

• Routine PCR
• High fidelity gene cloning

Specifications

2x GoldStar Best PCR Master Mix (with dye) is a convenient premixed 2x concentrated solution for PCR which include GoldStar Best DNA Polymerase, PCR Buffer, Mg2+, dNTPs, PCR Stabilizer and PCR Intensifier.

The polymerase has a 5´→3´ DNA polymerase and a 5´→3´ exonuclease activity. It also possesses 3´→5´ exonuclease (proofreading) activity. The polymerase has a higher amplification efficiency and lower mispairing rate than routine Taq DNA polymerase.

The GoldStar Best is a high reliable hotstart polymerase, which activity is inhibited at ambient temperatures by the chemical modificaiton. This prevents the formation of misprimed products and primer dimers at ambient temperatures. GoldStar Best Polymerase is activated by a 10 minutes, 95°C incubation. Optimized buffer system promotes the amplification of target fragment with high fidelity, high specificity, high amplification efficiency and high sensitivity.

GoldStar Best DNA Polymerase catalyzes the non-template directed addition of an adenine residue to the 3´-end of both strands of DNA molecules to make it suitable for TA cloning.

The amplification range of Es Taq is ~ 6 kb.

This PCR Master Mix contains dye, and can directly run electrophoresis after PCR reaction.

Shipping / Storage

Quality control

Manual (protocol)

PCR reaction system

Note: The recommended primer concentration for PCR is between 0.1-1.0 µM of each primer. The use of higher concentrations of primers can have higher amplification effect. Low primer concentration will generally ensure cleaner product and lower background.

PCR reaction conditions

Note:

• The recommended annealing temperature is about 5℃ below Tm of primers. If extra bands are observed, higher annealing temperatures should be considered. The absence of product indicates the need for a lower annealing temperature.
• PCR extension time is depended on the size of target gene sequence. GoldStar Best DNA polymerase is approximately 1 kb DNA / 60 seconds.
• The number of PCR cycles will basically depend on the downstream application of the PCR product.

This PCR Master Mix contains dye for electrophoresis. After PCR, directly load 5 µL of PCR product to agarose gel to run electrophoresis. No need to add loading buffer.