Also available at    &     VWR
Pure Plasmid Maxiprep Kit

Name

Pure Plasmid Maxiprep Kit

Cat. #

W0503-10
$99.00
(10 reactions)

Application

Sample Yield Elution Method DNA Quality
150 ~ 300 mL E. Coli 200μg~1.5mg plasmid DNA 1 ~ 3 mL Spin Column PCR, Enzyme digestion, Sequencing
This product is for research use only.

Description and features

This kit is based on a modified alkaline lysis method, with improved reagent formulation and silica membrane, which has high adsorption efficiency of the plasmid DNA. The proteins, genomes, RNA and other impurities can be removed after washing and an elution.

200 μg ~ 1.5 mg plasmid DNA can be purified from 100 ~ 300 mL overnight culture of E. Coli in LB medium with this kit.

The extracted DNA can be directly used for PCR, restriction enzyme digestion, sequencing, etc. No need to precipitate, concentrate or desalt.

Shipping / Storage

Ship at room temperature. Store RNase A or RNase A added buffer P1 at 4oC

Shelf Life

12 months

Manual (protocol)

  101Bio.com
  Pure Plasmid Maxiprep Kit

General Procedure

Pure Plasmid DNA Maxiprep Kit, Plasmid DNA purification Kit

Components

Components Amount Storage
Buffer P1 125 mL Room temperature; store at 4oC if RNase A is added.
Buffer P2 125 mL Room temperature
Buffer P3 125 mL Room temperature
Buffer PB 50 mL Room temperature
Buffer PW ( concentrate ) 25 mL x 2 Room temperature
Buffer EB 30 mL Room temperature
RNase A ( 10 mg/mL ) 1 mL x 2 4oC
Spin Column (Maxi) 10 Room temperatureC
Collection Tube 20 Room temperatureC
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