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Retroviral Packaging Mix

Name

Retroviral Packaging Mix

Cat. #

P905P
$439.00

Application

For packaging retrovirus. This product is for research use only.

Description

The Retroviral Packaging Mix provides a rapid and convenient method for producing high titers of replication-incompetent retroviruses. This Moloney Murine Leukemia Virus (MMLV)- based packaging system possesses the ideal host range required for your target cells. It is a ready-to-use retroviral packaging mix, which contains VSVG and produces high titer retroviruses in only 48 hours. Separate expression of these essential viral structural genes ensures safety and control over the recombinant constructs by minimizing the risks that arise during cell division and reducing the chance of producing replication-competent viruses.

This Mix is an optimized formulation for producing high-titer pantropic retrovirus.

  • • Achieve average titer of 107 infectious units/ml with transient transfection.
  • • Simple cotransfection of the retroviral packaging mix and the expression vector containing your gene of interest.
  • • Produce self-inactive and pantropic retrovirus.

Shipping / Storage

Ship at 4℃ or RT; Store at -20℃. This product is stable for 6 months when stored at -20℃. Freeze-thaw cycles should be minimized by dividing into single-use aliquots. Store aliquots in the -20℃ freezer until ready for use.

Shelf Life

6 months

Volume

200 µl

Manual (protocol)

Exosome Isolation Purity > 95%
Protein Extraction 1 min total protein, 40 min membrane protein
3D Cell Culture Gel 30% < Mkt Price
PCR Kits 50% < Mkt price
Beta-Hexosaminidase Activity Colorimetric Assay Fast and sensitive, High-throughput
Endotoxin-Free Plasmid Kits maxi, midi and mini-prep