Also available at    &     VWR
10-min. Tissue (Tumor) Dissociation / Single Cell Isolation Kit

Name

10-min. Tissue (Tumor) Dissociation / Single Cell Isolation Kit

Cat. #

P712-4
$149.00
(  4 reactions)    order from  Fisher
(Fisher Cat.# NC0899986)

P712-25
$399.00
(25 reactions)    order from  Fisher
(Fisher Cat.# NC0896869)

P712-50
$599.00
(50 reactions)    contact Fisher
Also can buy from :

Application

This kit is designed to isolate single cells from fresh/formaldehyde-fixed animal tissues (including tumor tissues), in less than 15 minutes.

Tissue can be dissociated to single cells for FACS, DNA/RNA/protein extraction or other cellular component isolation.

This kit can process 10 ~ 60 mg animal tissues pre reaction. The isolation is non-sterile.

This product is for research use only.

Description and features

This Tissue (Tumor) Dissociation / Single Cell Isolation Kit is designed to isolate single cells from fresh/formaldehyde-fixed animal tissues, in less than 10 minutes. The tissue dissociation / disaggregation buffers are formulated to gently disaggregate animal tissues. The buffers don’t contain any proteinases that may have adverse effects on cell surface marker detection.

  • Fast, < 15 minutes
  • Enzyme-free
  • Cell surface marker is maintained.

Shipping / Storage

Ship at room temperature, store at 4oC.

Shelf Life

12 months

Manual (protocol)

Components

Components Amount Storage
P712-4 P712-25 P712-50
Buffer A
(for non-fixed tissue)
2 mL 12.5 mL 25 mL 4oC
Buffer B
(for formaldehyde fixed tissue)
2 mL 12.5 mL 25 mL 4oC
Filter cartridges 4 25 50 Room temperature
Collection tubes with cap 4 25 50 Room temperature
Plastic rod 1 2 4 Room temperature
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