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101Bio WB Stripping Solution

Name

101Bio WB Stripping Solution

Cat. #

P5W3
$  99.00
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Application

This product is for maximum removal of antibodies from a membrane and preserving the integrity of the antigen in 12-15 minutes .

This product is for research use only.

Product Size

250 ml

Description

101Bio WB Stripping Solution (stripping buffer) is designed to rapidly strip antibodies bound to antigens on Western blotting membranes using chemiluminescent substrate. One of the major advantages of chemiluminescent detection is high sensitivity and the ability to strip reagents from a blot and re-probe the same blot. Re-probing Western blots are very useful for optimizing the blotting conditions such as testing different antibody dilution and membrane blocking conditions. The key to this process is to use conditions that cause the release of antibody from the antigen without causing a significant amount of antigen to be released from the membrane. During the stripping procedure, small amount of antigen is inevitably lost from the membrane. It is important to minimize this loss by stripping the antibody under gentle conditions.

101Bio WB stripping solution can achieve maximum removal of antibodies from a membrane and preserving the integrity of the antigen in 12-15 minutes without using harsh conditions such as boiling and treated with strong reducing agents. A blot can be stripped and re-probed multiple times to visualize multiple proteins or to optimize experimental conditions without the need for running multiple gels.

  • Ready-to-use
  • Short incubation time (12-15 minutes)
  • Gentle stripping conditions
  • long shelf life (2 years at RT)

Shipping / Storage

Ship and store at room temperature.

Shelf Life

24 months

Manual (protocol)

  101Bio.com
  WB Stripping Solution

Components

Components Amount Storage
101Bio WB Stripping Solution 250 ml Room temperature
WB Stripping Solution, stripping buffer
Protein Analysis Reagents
Name Cat. # Size Price Order
101Bio HRP Substrate Kit P5W1 30 ml /30 ml $  99.00
101Bio Antibody Enhancer P5W2 30 ml $  99.00
101Bio WB Stripping Solution P5W3 250 ml $  99.00
101Bio Ponceau S Stain P5W4 250 ml $  39.00
101Bio Peroxidase Suppressor P5W5 30 ml $  60.00
101Bio High Efficiency Protein Precipitation kit P5W6 30 ml /30 ml $129.00
101Bio Red Blood Cell Lysis Buffer P5W7 250 ml $  98.00
101Bio WB Blocking Solution P5W8 250 ml $  99.00
101Bio Denaturing Protein Solubilization Reagent P5W9 10 ml $  30.00
101Bio Non-Denatured Protein Solubilization Reagent P5W10 10 ml $  30.00
101Bio Protein Solubilization Reagent for MS P5W11 2 ml $  30.00
101Bio SDS-Remover P5W12 10 ml $  40.00
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