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Nuclei and Cytosol Isolation Kit for Adipose Tissues


Nuclei and Cytosol Isolation Kit for Adipose Tissues

Cat. #

$   389.00
(20 reactions)

Researchers also buy our Protein Analysis Reagents (HRP, Antibody enhancer, WB stripping solution, etc.)
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This kit provides a very rapid and simple method for obtaining high purity nuclei from adipose tissues.

This product is for research use only.

Product Size

20 reactions


Adipocytes are the major energy storage sites in the body and they also have critical endocrine functions. Therefore, understanding the development and function of adipocytes are essential to understanding metabolic homeostasis under physiological and pathological conditions. Fractionation of cellular components into nucleus and cytosolic fraction is a common practice in the lab. However, when it comes to adipocytes, separation of these two fractions are much more difficult because of high concentration of lipid droplet and low protein content of adipocytes. Methods reported in the literature are tedious and time consuming and as much as 50 grams of tissue are required. This kit provides a very rapid and simple method for obtaining high purity nuclei from adipose tissues and most important of all, only milligram amounts of tissues are required making it possible to isolate nuclei and cytosol from small animal and biopsy samples.

  • Fast - 30 minutes
  • Only require 120-150 mg tissue

Shipping / Storage

Ship and store at ambient temperature

Shelf Life

12 months

Manual (protocol)


Components Amount Storage
N/C Buffer 15 mL     Room temperature
1.5 ml Tube 20 Room temperature
Pestles for 1.5 ml tube 2 Room temperature
Filter cartridges with collection tubes 20 Room temperature


  • Make sure your freezer is about -20oC. Otherwise the result will not be good.
  • For determination of protein concentration, BCA kit is recommended.
  • 3. To study protein phosphorylation, phosphatase inhibitors (such as PhosStop from Roche) should be added to N/C buffer prior to use. The use of protease inhibitor cocktails is optional. If you are interested in use of cytosolic protein for downstream experiment, protease inhibitors should be added to N/C Buffer prior to use.
  • This protocol is developed and validated by 101Bio’s OEM partner. Spin column based protein extraction and cell fractionation technologies were developed by 101Bio’s OEM partner.
Nuclei and Cytosol Isolation Kit for Adipose Tissues
Adipose nuclear protein
Protein Analysis Reagents
Name Cat. # Size Price Order
101Bio HRP Substrate Kit P5W1 30 ml /30 ml $  99.00
101Bio Antibody Enhancer P5W2 30 ml $  129.00
101Bio WB Stripping Solution P5W3 250 ml $  129.00
101Bio Ponceau S Stain P5W4 250 ml $  59.00
101Bio Peroxidase Suppressor P5W5 30 ml $  89.00
101Bio High Efficiency Protein Precipitation kit P5W6 30 ml /30 ml $129.00
101Bio Red Blood Cell Lysis Buffer P5W7 250 ml $  109.00
101Bio WB Blocking Solution P5W8 250 ml $  109.00
101Bio Denaturing Protein Solubilization Reagent P5W9 10 ml $  49.00
101Bio Non-Denatured Protein Solubilization Reagent P5W10 10 ml $  49.00
101Bio Protein Solubilization Reagent for MS P5W11 2 ml $  49.00
101Bio SDS-Remover P5W12 10 ml $  79.00
101Bio Albumin Depletion Reagent for Plasma and Serum P5W13 10 ml $  109.00
101Bio Protein A+G Agarose Beads W0349 1 ml $  99.00
101Bio Protease Inhibitor Cocktail (100x) W2200 1 ml $  49.00
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