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Endosome Isolation Kit

Name

Endosome Isolation Kit

Cat. #

P528
$   469.00
(  20 reactions)


Researchers also buy our Protein Analysis Reagents (HRP, Antibody enhancer, WB stripping solution, etc.)
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Application

Rapid and simple endosome isolation using spin-column, requiring smaller amount of cells or tissues.
This product is for research use only.

Product Size

20 reactions

Description

Early endosomes (EE) provide the starting point for late endosome maturation. The EEs are mainly derived from primary endocytic vesicles that fuse with each other. EEs receive endocytic cargos not only through the clathrin-mediated pathway but several other pathways. In addition to their roles in normal cell physiology, endocytic processes play a key role in many diseases such as Alzheimer’s disease and inherited lysosomal storage diseases. Traditional methods for isolating endosomes are based on density gradient ultracentrifugation. The protocol requires large amount of starting material and the methods are tedious and time consuming.

101Bio Endosome Isolation Kit provides a spin-column based novel endosome isolation technology which is rapid and simple. It only requires smaller amount of cultured cells or milligram amounts of tissues. This kit can precipitate and significantly enrich early endosomes from cultured cells or tissues. The availability of the kit should facilitate the research in the field.

  • Fast - 20 minutes hands-on

Shipping / Storage

Ship at ambient temperature, store this kit at room temperature except Buffer A at 4oC

Shelf Life

12 months

Manual (protocol)

Components

Components Amount Storage
Buffer A 15 mL     4oC
Buffer B 15 mL     4oC
Plastic rods 2 Room temperature
Filter cartridges with collection tubes 20 Room temperature
Tissue dissociation beads 2.5 g Room temperature

Remarks

  • Read the entire procedures carefully. Chill protein extraction filter cartridge with collection tube on ice prior to use.
  • All centrifugation steps should be performed at 4oC in a cold room or in a refrigerated microfuge.
  • To study protein phosphorylation, phosphatase inhibitors (such as PhosStop from Roche) should be added to Buffer A prior to use. The use of protease inhibitor cocktails is optional.
  • It is recommended to use BCA Protein Assay Kit for determination of protein concentration.
  • This protocol is developed and validated by 101Bio’s OEM partner. Spin column based protein extraction and cell fractionation technologies were developed by 101Bio’s OEM partner.
exosome vs endosome
Protein Analysis Reagents
Name Cat. # Size Price Order
101Bio HRP Substrate Kit P5W1 30 ml /30 ml $  99.00
101Bio Antibody Enhancer P5W2 30 ml $  99.00
101Bio WB Stripping Solution P5W3 250 ml $  99.00
101Bio Ponceau S Stain P5W4 250 ml $  39.00
101Bio Peroxidase Suppressor P5W5 30 ml $  60.00
101Bio High Efficiency Protein Precipitation kit P5W6 30 ml /30 ml $129.00
101Bio Red Blood Cell Lysis Buffer P5W7 250 ml $  98.00
101Bio WB Blocking Solution P5W8 250 ml $  99.00
101Bio Denaturing Protein Solubilization Reagent P5W9 10 ml $  30.00
101Bio Non-Denatured Protein Solubilization Reagent P5W10 10 ml $  30.00
101Bio Protein Solubilization Reagent for MS P5W11 2 ml $  30.00
101Bio SDS-Remover P5W12 10 ml $  40.00
101Bio Albumin Depletion Reagent for Plasma and Serum P5W13 10 ml $  99.00
101Bio Protein A+G Agarose Beads W0349 1 ml $  99.00
101Bio Protease Inhibitor Cocktail (100x) W2200 1 ml $  49.00
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