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Protein Extraction Kit for Fixed and Embedded Tissues (FFPE)

Name

Protein Extraction Kit for Fixed and Embedded Tissues (FFPE)

Cat. #

P525-20
$299.00
(20 rxn)

Researchers also buy our Protein Analysis Reagents (HRP, Antibody enhancer, WB stripping solution, etc.)
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Application

This kit can quickly extract protein from Formalin-Fixed and Paraffin-Embedded (FFPE) tissue without organic solvent deparaffinization. The whole procedure is less than 1 hour with a protein yield of 2-3mg/ml.

Extracted proteins can be used for many downstream applications such as SDS-PAGE, Western blotting ELISA, immunoprecipitation and proteomic studies.

This product is for research use only.

Description

FFPE tissues are widely available and serve as a potential rich source for biomedical research. However, protein extraction from FFPE tissue is very challenging due to formalin mediated molecular cross-linking. Traditional methods for protein extraction from FFPE tissues involve repeated deparaffinization and rehydration using organic solvents followed by protein extraction at high temperature with or without optional sonication step. These methods, though relatively effective in some cases, are tedious and time consuming (3-4 h).

This kit can quickly extract protein from Formalin-Fixed and Paraffin-Embedded (FFPE) tissue without organic solvent deparaffinization. The whole procedure is less than 1 hour with a protein yield of 2-3mg/ml.

Extracted proteins can be used for many downstream applications such as SDS-PAGE, Western blotting ELISA, immunoprecipitation and proteomic studies.

  • Fast - less than 1 hour

Shipping / Storage

Ship and store at room temperature.

Shelf Life

12 months

Manual (protocol)

Components
(Cat.#: P522-20)

Components Amount Storage
Buffer A (extraction buffer) 20 ml Room temperature
Buffer B (10X denaturing buffer) 20 ml Room temperature
Microfuge tube (1.5 ml) 20 Room temperature
Pestles for 1.5 mL tube 2 Room temperature

Remarks

  • The yield and resulting protein profile are fixation time and sample age dependent. Longer fixation time (>48h) and aging samples are expected to yield lower protein concentration.
  • Protease inhibitor cocktail is recommended to be added to Buffer B after the buffer is transferred to 1.5 ml microfuge tube (see step 3 below).
  • For determination of protein concentration, detergent-compatible BCA kit is recommended because the extraction buffer (Buffer B) contains 1% SDS. To study protein phosphorylation, phosphatase inhibitors should be added to buffer B prior to use.
            Protein Extraction Kit for Fixed and Embedded Tissues, FFPE
Protein yield and profile of 20, 40 and 60 minutes extraction using P525 kit.
The protein was extracted from mouse kidney tissue fixed with formalin for 7 days.
protein extraction method, FFPE protein extraction
Proteins extracted from FFPE (formalin-fixed and embedded, 7 days) mouse tissues using our P525 kit.
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