Also available at    &     VWR
Total Protein Extraction Kit for
Adipose Tissues and Cultured Adipocytes


Total Protein Extraction Kit for Adipose Tissues and Cultured Adipocytes

Cat. #

$   119.00
(  4 reactions)    order from  Fisher
(Fisher Cat.# NC1261707)

$   289.00
(20 reactions)    order from  Fisher
(Fisher Cat.# NC1290672)

$ 1099.00
(80 reactions)    contact Fisher

Researchers also buy our Protein Analysis Reagents (HRP, Antibody enhancer, WB stripping solution, etc.)
Also can buy from :


Fast extract protein from white and brown adipose tissue (WAT/BAT), and cultured adpocytes in 20 minute with high yield (2~3 mg/mL).

Extracted protein can be used in 2D gel analysis, ELISA, SDS-PAGE, immunoblottings, immunoprecipitation, enzyme activity assays, and other applications.

This product is for research use only.


Adipose tissue has been recognized as an important endocrine and inflammation organ in addition to its energy storage function. Isolation and analysis of proteins from adipose tissues are increasingly critical for understanding many physiological/pathological conditions. However isolation and analysis of WAT (white adipose tissue) and BAT (brown adipose tissue) are technically very challenging due to their high lipid and low protein contents. The water-oil emulsion present in biological sample is notoriously difficult to separate.

We have developed a novel technology to rapidly extract protein from water-oil emulsion derived from adipose tissue homogenate. A filter with unique surface property and a specially formulated detergent-free extraction buffer are employed.

The total proteins isolated are un-biased representation of cellular proteins in the tissue. The extracted proteins concentration is very high (2-3 mg/ml) as compared to other methods.

  • Fast - as quick as 15 to 20 minutes

Shipping / Storage

Ship at room temperature. Store Buffer A at 4oC, and store the rest of the kit at RT

Shelf Life

12 months

Manual (protocol)

(Cat.#: P522-20)

Components Amount Storage
Buffer A (extraction buffer) 15 mL     4oC
Buffer B (10X denaturing buffer) 1.5 mL Room temperature
Buffer C (10X non-denaturing buffer) 1.5 mL Room temperature
Microfuge tube (1.5 mL) 20 Room temperature
Pestles for 1.5 mL tube 2 Room temperature
Protein extraction filter cartridges with collection tubes 20 Room temperature
Protein Extraction Powder 2g Room temperature


  • Protease inhibitors is not necessary prior to extraction. However if downstream application takes significant amounts of time or the protein extract will be stored for longer period of time, addition of protease inhibitors to buffer A is recommended.
  • For determination of protein concentration, BCA kit (Pierce) is recommended.
  • To study protein phosphorylation, phosphatase inhibitors (such as PhosStop from Roche) should be added to buffer A prior to use.
  • This protocol is developed and validated by 101Bio’s OEM partner. Spin column based protein extraction and cell fractionation technologies were developed by 101Bio’s OEM partner.
Total Protein Extraction Kit for Adipose Tissues Adipocytes
Total Protein Extraction Kit for Adipose Tissues Adipocytes
Total Protein Extraction Kit for Adipose Tissues Adipocytes
Protein Analysis Reagents
Name Cat. # Size Price Order
101Bio HRP Substrate Kit P5W1 30 ml /30 ml $  99.00
101Bio Antibody Enhancer P5W2 30 ml $  129.00
101Bio WB Stripping Solution P5W3 250 ml $  129.00
101Bio Ponceau S Stain P5W4 250 ml $  59.00
101Bio Peroxidase Suppressor P5W5 30 ml $  89.00
101Bio High Efficiency Protein Precipitation kit P5W6 30 ml /30 ml $129.00
101Bio Red Blood Cell Lysis Buffer P5W7 250 ml $  109.00
101Bio WB Blocking Solution P5W8 250 ml $  109.00
101Bio Denaturing Protein Solubilization Reagent P5W9 10 ml $  49.00
101Bio Non-Denatured Protein Solubilization Reagent P5W10 10 ml $  49.00
101Bio Protein Solubilization Reagent for MS P5W11 2 ml $  49.00
101Bio SDS-Remover P5W12 10 ml $  79.00
101Bio Albumin Depletion Reagent for Plasma and Serum P5W13 10 ml $  109.00
101Bio Protein A+G Agarose Beads W0349 1 ml $  99.00
101Bio Protease Inhibitor Cocktail (100x) W2200 1 ml $  49.00
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