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Total Protein Extraction Kit for
Adipose Tissues and Cultured Adipocytes

Name

Total Protein Extraction Kit for Adipose Tissues and Cultured Adipocytes

Cat. #

P522-4
$   119.00
(  4 reactions)    order from  Fisher
(Fisher Cat.# NC1261707)

P522-20
$   289.00
(20 reactions)    order from  Fisher
(Fisher Cat.# NC1290672)

P522-80
$ 1099.00
(80 reactions)    contact Fisher
Also can buy from :

Application

Fast extract protein from white and brown adipose tissue (WAT/BAT), and cultured adpocytes in 20 minute with high yield (2~3 mg/mL).

Extracted protein can be used in 2D gel analysis, ELISA, SDS-PAGE, immunoblottings, immunoprecipitation, enzyme activity assays, and other applications.

This product is for research use only.

Description

Adipose tissue has been recognized as an important endocrine and inflammation organ in addition to its energy storage function. Isolation and analysis of proteins from adipose tissues are increasingly critical for understanding many physiological/pathological conditions. However isolation and analysis of WAT (white adipose tissue) and BAT (brown adipose tissue) are technically very challenging due to their high lipid and low protein contents. The water-oil emulsion present in biological sample is notoriously difficult to separate.

We have developed a novel technology to rapidly extract protein from water-oil emulsion derived from adipose tissue homogenate. A filter with unique surface property and a specially formulated detergent-free extraction buffer are employed.

The total proteins isolated are un-biased representation of cellular proteins in the tissue. The extracted proteins concentration is very high (2-3 mg/ml) as compared to other methods.

  • Fast - as quick as 15 to 20 minutes

Shipping / Storage

Ship at room temperature. Store Buffer A at 4oC, and store the rest of the kit at RT

Shelf Life

12 months

Manual (protocol)

Components
(Cat.#: P522-20)

Components Amount Storage
Buffer A (extraction buffer) 15 mL     4oC
Buffer B (10X denaturing buffer) 1.5 mL Room temperature
Buffer C (10X non-denaturing buffer) 1.5 mL Room temperature
Microfuge tube (1.5 mL) 20 Room temperature
Pestles for 1.5 mL tube 2 Room temperature
Protein extraction filter cartridges with collection tubes 20 Room temperature
Protein Extraction Powder 2g Room temperature

Remarks

  • Protease inhibitors is not necessary prior to extraction. However if downstream application takes significant amounts of time or the protein extract will be stored for longer period of time, addition of protease inhibitors to buffer A is recommended.
  • For determination of protein concentration, BCA kit (Pierce) is recommended.
  • To study protein phosphorylation, phosphatase inhibitors (such as PhosStop from Roche) should be added to buffer A prior to use.
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