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Protein Extraction Kit for Hair, Nail, Wool and Horn

Name

Protein Extraction Kit for Hair, Nail, Wool and Horn

Cat. #

P521-4
$   149.00
(  4 rxn)

P521-20
$   299.00
(20 rxn)

P521-80
$ 1099.00
(80 rxn)

Researchers also buy our Protein Analysis Reagents (HRP, Antibody enhancer, WB stripping solution, etc.)
Also can buy from :

Application

This kit is designed to effectively extract proteins from keratinized animal tissues without using strong denaturants (such as urea, thiourea, guanidine hydrochloride or a combination of these).

The extracted protein can be directly loaded onto SDS-PAGE gel without concentrating.

The extracted protein concentration is 1-2 mg/mL.

The extracted protein can be used in SDS-PAGE, immunoblottings, ELISA and other applications.

This product is for research use only.

Description

Proteins account for about 80% of keratinized animal tissues that include but not limited to hair, nail, horn and wool. These tissues contain mainly hard α-keratins made of several distinctive types of protein with molecular weight ranging from 10-135 Kda. Majority of the proteins are ranging from 10-65 Kda. Several methods have been reported for protein extraction from keratinized animal tissues. The extraction buffer usually contains high concentration of urea, thiourea, guanidine hydrochloride or a combination of these strong denaturants. Protein extraction with high concentration denaturants is relatively effective but the concentration of extracted protein is usually low due to larger extraction buffer/tissue ratio. Extracted proteins need to be concentrated prior to further analysis. The presence of high concentration of denaturants may also interfere with downstream application.

We have developed a novel technology to extract protein from keratinized tissues without using those strong denaturants.

The extracted proteins concentration is higher (1-2 mg/mL) than other methods.

The extraction buffer of this kit contains 0.5% SDS and other chemicals.

Shipping / Storage

Ship and store at room temperature.

Shelf Life

12 months

Manual (protocol)

Components
(Cat.#: P521-20)

Components Amount Storage
Buffer A 15 mL Room temperature
Buffer B 1.5 mL Room temperature
Protein extraction filter cartridges 20 Room temperature
Collection tubes 20 Room temperature

Remarks

  • Protease inhibitors is not necessary prior to extraction. However if downstream application takes significant amounts of time or the protein extract will be stored for longer period of time, addition of protease inhibitors to buffer A is recommended.
  • For determination of protein concentration, BCA kit (Pierce) is recommended.
  • To study protein phosphorylation, phosphatase inhibitors (such as PhosStop from Roche) should be added to buffer A prior to use.
  • This protocol is developed and validated by 101Bio’s OEM partner. Spin column based protein extraction and cell fractionation technologies were developed by 101Bio’s OEM partner.
Protein Analysis Reagents
Name Cat. # Size Price Order
101Bio HRP Substrate Kit P5W1 30 ml /30 ml $  99.00
101Bio Antibody Enhancer P5W2 30 ml $  99.00
101Bio WB Stripping Solution P5W3 250 ml $  99.00
101Bio Ponceau S Stain P5W4 250 ml $  39.00
101Bio Peroxidase Suppressor P5W5 30 ml $  60.00
101Bio High Efficiency Protein Precipitation kit P5W6 30 ml /30 ml $129.00
101Bio Red Blood Cell Lysis Buffer P5W7 250 ml $  98.00
101Bio WB Blocking Solution P5W8 250 ml $  99.00
101Bio Denaturing Protein Solubilization Reagent P5W9 10 ml $  30.00
101Bio Non-Denatured Protein Solubilization Reagent P5W10 10 ml $  30.00
101Bio Protein Solubilization Reagent for MS P5W11 2 ml $  30.00
101Bio SDS-Remover P5W12 10 ml $  40.00
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54.162.227.37