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Protein Extraction Kit from Gel Slices

Name

8-min. Protein Extraction Kit from Gel Slices

Cat. #

P519-20
$199.00
(20 reactions)    order from  Fisher
(Fisher Cat.# NC0887243)

P519-80
$729.00
(80 reactions)    contact Fisher

Researchers also buy our Protein Analysis Reagents (HRP, Antibody enhancer, WB stripping solution, etc.)
Also can buy from :

Application

8 minutes gel slice protein recovery at high yield.

Extracted proteins can be used for MALD-MS analysis, immunization of animals, protein-protein interaction and protein-nucleic acid interaction studies etc.

This product is for research use only.

Product Size

P519-20: 20 reactions;    P519-80: 80 reactions

Description

This kit is for fast extraction of proteins from gel slices.

  • Fast - 8 minutes
  • No overnight waiting
  • No need for special device
  • High efficient for 10~200 kDa protein
  • high concentration - 10 ~ 200 uL final volume - Multiple gel pieces can be processed in one tube.
  • Can be detergent-free

Shipping / Storage

Ship and store at room temperature.

Shelf Life

12 months

Manual (protocol)

  101Bio.com
  8-min. Protein Extraction Kit from Gel Slices

Components
(Cat.#: P519-20)

Components Amount Storage
Filter Cartridges 20 Room temperature
Collection tubes with cap 20 Room temperature
Microfuge tubes (200 uL) 20 Room temperature
Extraction powder 2g Room temperature
Micro pestle 4 Room temperature

Remarks

  • The choice of elution buffer depends on how gel is stained and the downstream applications.
  • If the stain contains fixing agent such as methanol and acetic acid, an elution buffer containing 0.1-0.5% SDS or acid labile surfactants is recommended.
  • If the gel is un-stained or negatively stained the protein can be eluted with ddH2O or an elution buffer containing formic acid/water/2-propanol (1:3:2 v/v/v).
Protein Extraction Kit from Gel Slices, gel slice protein recovery kit
Protein Analysis Reagents
Name Cat. # Size Price Order
101Bio HRP Substrate Kit P5W1 30 ml /30 ml $  99.00
101Bio Antibody Enhancer P5W2 30 ml $  99.00
101Bio WB Stripping Solution P5W3 250 ml $  99.00
101Bio Ponceau S Stain P5W4 250 ml $  39.00
101Bio Peroxidase Suppressor P5W5 30 ml $  60.00
101Bio High Efficiency Protein Precipitation kit P5W6 30 ml /30 ml $129.00
101Bio Red Blood Cell Lysis Buffer P5W7 250 ml $  98.00
101Bio WB Blocking Solution P5W8 250 ml $  99.00
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