Also available at    &     VWR
Protein Extraction Kit from Gel Slices

Name

8-min. Protein Extraction Kit from Gel Slices

Cat. #

P519-20
$199.00
(20 reactions)    order from  Fisher
(Fisher Cat.# NC0887243)

P519-80
$729.00
(80 reactions)    contact Fisher

Researchers also buy our Protein Analysis Reagents (HRP, Antibody enhancer, WB stripping solution, etc.)
Also can buy from :

Application

8 minutes gel slice protein recovery at high yield.

Extracted proteins can be used for MALD-MS analysis, immunization of animals, protein-protein interaction and protein-nucleic acid interaction studies etc.

This product is for research use only.

Product Size

P519-20: 20 reactions;    P519-80: 80 reactions

Description

This kit is for fast extraction of proteins from gel slices.

  • Fast - 8 minutes
  • No overnight waiting
  • No need for special device
  • High efficient for 10~200 kDa protein
  • high concentration - 10 ~ 200 uL final volume - Multiple gel pieces can be processed in one tube.
  • Can be detergent-free

Shipping / Storage

Ship and store at room temperature.

Shelf Life

12 months

Manual (protocol)

  101Bio.com
  8-min. Protein Extraction Kit from Gel Slices

Components
(Cat.#: P519-20)

Components Amount Storage
Filter Cartridges 20 Room temperature
Collection tubes with cap 20 Room temperature
Microfuge tubes (200 uL) 20 Room temperature
Extraction powder 2g Room temperature
Micro pestle 4 Room temperature

Remarks

  • The choice of elution buffer depends on how gel is stained and the downstream applications.
  • If the stain contains fixing agent such as methanol and acetic acid, an elution buffer containing 0.1-0.5% SDS or acid labile surfactants is recommended.
  • If the gel is un-stained or negatively stained the protein can be eluted with ddH2O or an elution buffer containing formic acid/water/2-propanol (1:3:2 v/v/v).
  • This protocol is developed and validated by 101Bio’s OEM partner. Spin column based protein extraction and cell fractionation technologies were developed by 101Bio’s OEM partner.
Protein Extraction Kit from Gel Slices, gel slice protein recovery kit
Protein Analysis Reagents
Name Cat. # Size Price Order
101Bio HRP Substrate Kit P5W1 30 ml /30 ml $  99.00
101Bio Antibody Enhancer P5W2 30 ml $  99.00
101Bio WB Stripping Solution P5W3 250 ml $  99.00
101Bio Ponceau S Stain P5W4 250 ml $  39.00
101Bio Peroxidase Suppressor P5W5 30 ml $  60.00
101Bio High Efficiency Protein Precipitation kit P5W6 30 ml /30 ml $129.00
101Bio Red Blood Cell Lysis Buffer P5W7 250 ml $  98.00
101Bio WB Blocking Solution P5W8 250 ml $  99.00
101Bio Denaturing Protein Solubilization Reagent P5W9 10 ml $  30.00
101Bio Non-Denatured Protein Solubilization Reagent P5W10 10 ml $  30.00
101Bio Protein Solubilization Reagent for MS P5W11 2 ml $  30.00
101Bio SDS-Remover P5W12 10 ml $  40.00
101Bio Protein A+G Agarose Beads W0349 1 ml $  99.00
101Bio Protease Inhibitor Cocktail (100x) W2200 1 ml $  49.00
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