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Plant Microsomal Membrane Extraction Kit

Name

Plant Microsomal Membrane Extraction Kit

Cat. #

P518-25
$399.00
(25 reactions)

Researchers also buy our Protein Analysis Reagents (HRP, Antibody enhancer, WB stripping solution, etc.)
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Application

Rapidly extract microsomal fraction from small amount of starting material (200 mg).

This product is for research use only.

Description

Plant microsomal fraction is believed to be enriched for plasma membranes, endoplasmic reticulum, Golgi apparatus, vacuolar membranes and other components of membrane system. Traditional microsomal fraction isolation method requires large amount of starting material and employs tedious ultracentrifugation steps. This kit offers a simple, rapid and user friendly approach for microsomal membrane extraction using small amount of starting material (200 mg). Water soluble cytosolic proteins are removed during the procedures and water insoluble microsomal fraction, especially plasma membrane fraction, is extracted with optimized buffers in a table-top microcentrifuge.

  • Simple and user friendly - no ultracentrifugation
  • No need liquid nitrogen
  • 1 hour protocol
  • Small amount of starting material – 200 mg
  • Native microsomal proteins
  • High yield – 100 to 200 µg/sample

Result

plant microsomal fraction extraction

Click to see full size image.

Shipping / Storage

Ship at room temperature. Store at -20℃

Shelf Life

12 months

Manual (protocol)

  101Bio.com
  Plant Microsomal Membrane Extraction Kit

Components

Components Amount Storage
Buffer A 12.5 mL -20℃
Buffer B 7.5 mL -20℃
Filter Cartridges 25 Room temperature
Collection tubes with cap 25 Room temperature
Plastic rods 2 Room temperature
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101Bio Non-Denatured Protein Solubilization Reagent P5W10 10 ml $  30.00
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