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10-min. Thick Cell Wall Microbes Detergent-Free Total Protein Extraction Kit

Name

10-min. Thick Cell Wall Microbes Detergent-Free Total Protein Extraction Kit

Cat. #

P516-50
$199.00
(50 reactions)

Researchers also buy our Protein Analysis Reagents (HRP, Antibody enhancer, WB stripping solution, etc.)
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Application

Protein extraction from cell wall-containing microbes is a frequent procedure in bio-research Labs. The methods for protein extraction from these microbes are usually harsh, tedious and not sufficiently reliable. P516-50 provides a detergent-free, rapid and gentle way for extracting proteins from microbes with thick and strong cell walls. These microbes include but not limited to yeast, filamentous fungus, gram positive and negative bacteria, insect eggs and microalgae. This kit contains optimized detergent-free and EDTA-free protein extraction buffers. The whole procedure takes less than 10 minutes to complete and the protein yield is in the range of 2-4 mg/ml with un-biased protein representation. This kit features a single tube protocol and works well for both log phase and stationary phase microbes. The materials provided are enough for 50 extractions.

Proteins extracted with this kit can be used for many downstream applications such as SDSPAGE analysis, Western blotting, IP. ELISA and enzyme activity assays and proteomic analysis. The buffers are compatible with IMAC resins for his-tagged protein purification.

This product is for research use only.

Description and features

Protein extraction from cell wall-containing microbes is a frequent procedure in bio-research Labs. The methods for protein extraction from these microbes are usually harsh, tedious and not sufficiently reliable. P516-50 provides a detergent-free, rapid and gentle way for extracting proteins from microbes with thick and strong cell walls. These microbes include but not limited to yeast, filamentous fungus, gram positive and negative bacteria, insect eggs and microalgae. This kit contains optimized detergent-free and EDTA-free protein extraction buffers. The whole procedure takes less than 10 minutes to complete and the protein yield is in the range of 2-4 mg/ml with un-biased protein representation. This kit features a single tube protocol and works well for both log phase and stationary phase microbes.

  • Fast - less than 10 minutes
  • High yield - 2 ~ 4 mg/mL
  • Full range of yeast proteins can be extracted without bias.
  • Detergent-free and EDTA-free

Shipping / Storage

Ship and store at room temperature.

Shelf Life

12 months

Manual (protocol)

  101Bio.com
  Thick Cell Wall Microbes Detergent-Free Total Protein Extraction Kit

Components

Components Amount Storage
Buffer A 20 mL Room temperature
Buffer B 20 mL Room temperature
Protein extraction powder 5g Room temperature
Pestles for 1.5 ml microcentrifuge tube 4 Room temperature
1.5 ml microcentrifuge tube 50 Room temperature

Remarks

  • Read the entire manual carefully. The use of protease inhibitors is optional for this kit. If downstream application takes significant amounts of time or the protein extract will be stored for longer period of time, addition of protease inhibitor to the extraction buffer is recommended.
  • For determination of protein concentration, BCA kit (Pierce) is recommended.
  • To study protein phosphorylation, phosphatase inhibitors (such as PhosStop from Roche) should be added to the buffer(s) prior to use.
  • This protocol is developed and validated by 101Bio’s OEM partner. Spin column based protein extraction and cell fractionation technologies were developed by 101Bio’s OEM partner.

Thick Cell Wall Microbes Detergent-Free Total Protein Extraction Kit

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101Bio Antibody Enhancer P5W2 30 ml $  99.00
101Bio WB Stripping Solution P5W3 250 ml $  99.00
101Bio Ponceau S Stain P5W4 250 ml $  39.00
101Bio Peroxidase Suppressor P5W5 30 ml $  60.00
101Bio High Efficiency Protein Precipitation kit P5W6 30 ml /30 ml $129.00
101Bio Red Blood Cell Lysis Buffer P5W7 250 ml $  98.00
101Bio WB Blocking Solution P5W8 250 ml $  99.00
101Bio Denaturing Protein Solubilization Reagent P5W9 10 ml $  30.00
101Bio Non-Denatured Protein Solubilization Reagent P5W10 10 ml $  30.00
101Bio Protein Solubilization Reagent for MS P5W11 2 ml $  30.00
101Bio SDS-Remover P5W12 10 ml $  40.00
101Bio Albumin Depletion Reagent for Plasma and Serum P5W13 10 ml $  99.00
101Bio Protein A+G Agarose Beads W0349 1 ml $  99.00
101Bio Protease Inhibitor Cocktail (100x) W2200 1 ml $  49.00
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