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10-min. Thick Cell Wall Microbes Total Protein Extraction Kit

Name

10-min. Thick Cell Wall Microbes Total Protein Extraction Kit

Cat. #

P515-50
$199.00
(50 reactions)

Researchers also buy our Protein Analysis Reagents (HRP, Antibody enhancer, WB stripping solution, etc.)
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Application

This kit provides an instrument-free, rapid and gentle way for extracting proteins from microbes with thick and strong cell walls.

These microbes include but not limited to yeast, filamentous fungus, gram positive and negative bacteria, insect eggs and microalgae.

The whole procedure takes less than 10 min to complete and the protein yield is in the range of 2-5 mg/ml

The extracted proteins can be used for SDS-PAGE analysis, Western blotting, IP, ELISA, enzyme activity assays and proteomic analysis. The buffers are compatible with IMAC resins for his-tagged protein purification.

This product is for research use only.

Description and features

The methods for protein extraction from thick cell wall microbes (including yeast, filamentous fungus, gram positive and negative bacteria, insect eggs and microalgae, etc.) are usually harsh, tedious and not sufficiently reliable.

This Thick Cell Wall Microbes Total Protein Extraction Kit provides an instrument-free, rapid and gentle way for extracting proteins from these microbes.

Our kit does not use harsh conditions, such as 8M urea, glass bead lysis using an instrument, or boiling the sample with alkaline etc.. This kit features a simple protocol, full range of yeast proteins are extracted without bias (unlike other kits, proteins in certain molecular weight range are preferentially extracted). The kit works well for both log phase and stationary phase microbes.

  • Fast - less than 10 minutes
  • High yield - 2 ~ 5 mg/mL
  • Full range of yeast proteins can be extracted without bias.

Shipping / Storage

Ship and store at room temperature.

Shelf Life

12 months

Manual (protocol)

  101Bio.com
  Thick Cell Wall Microbes Total Protein Extraction Kit

Components

Components Amount Storage
Denaturing buffer 20 mL Room temperature
Native buffer 20 mL Room temperature
Protein extraction powder 5g Room temperature
Pestles for 1.5 ml microcentrifuge tube 4 Room temperature
1.5 ml microcentrifuge tube 50 Room temperature

Remarks

  • Read the entire manual carefully. The use of protease inhibitors is optional for this kit. If downstream application takes significant amounts of time or the protein extract will be stored for longer period of time, addition of protease inhibitor to the extraction buffer is recommended.
  • For determination of protein concentration, BCA kit (Pierce) is recommended.
  • To study protein phosphorylation, phosphatase inhibitors (such as PhosStop from Roche) should be added to the buffer(s) prior to use.

S. cereviseae, filamentus fungus and L. Lactis (Gram+) protein extraction

Thick Cell Wall Microbes Total Protein Extraction Kit

SDS-PAGE (12%) profile of extracted proteins from different microbes.

Protein Analysis Reagents
Name Cat. # Size Price Order
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101Bio Antibody Enhancer P5W2 30 ml $  99.00
101Bio WB Stripping Solution P5W3 250 ml $  99.00
101Bio Ponceau S Stain P5W4 250 ml $  39.00
101Bio Peroxidase Suppressor P5W5 30 ml $  60.00
101Bio High Efficiency Protein Precipitation kit P5W6 30 ml /30 ml $129.00
101Bio Red Blood Cell Lysis Buffer P5W7 250 ml $  98.00
101Bio WB Blocking Solution P5W8 250 ml $  99.00
101Bio Denaturing Protein Solubilization Reagent P5W9 10 ml $  30.00
101Bio Non-Denatured Protein Solubilization Reagent P5W10 10 ml $  30.00
101Bio Protein Solubilization Reagent for MS P5W11 2 ml $  30.00
101Bio SDS-Remover P5W12 10 ml $  40.00
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