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10-min. Histone/DNA Binding Protein Extraction Kit  (Cells)

Name

10-min. Histone/DNA Binding Protein Extraction Kit (Cells)

Cat. #

P514-4
$129.00
( 4 reactions)

P514-25
$279.00
(25 reactions)

P514-50
$499.00
(50 reactions)
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Application

High efficient extraction for both histones and other DNA binding proteins (regardless of binding affinity) from cultured cells or cells isolated from animal tissues, in less than 10 minute.

The extracted protein can be used for SDS-PAGE, immunoblottings, ELISA, IP, and protein localization and modification studies.

This product is for research use only.

Description and features

Histones and DNA binding proteins play an important role in chromosome organization, gene regulation and posttranslational modification. Our Histone/DNA Binding Protein Extraction Kit is designed to quickly extract histones and other DNA binding proteins both at high efficiency from cultured cells and cells isolated from animal tissues, in less than 10 minutes.

Many other commercial kits that employ acid or high salt extraction methods, selectively extract basic proteins and good internal control is usually lost. Our P514 kit extracts histone proteins through a completely different mechanism. This kit has equally high efficiency in extracting both histones and other DNA binding proteins that can be used for internal controls for histone modification analysis.

P514 can extract histones from 0.5 to 5 million cells in less than 10 min with high protein yield (1~2.5 mg/ml) making it the fastest and most robust histone extraction kit available in the market.

  • Fast - less than 10 minutes
  • High yield - 1~2.5 mg/mL
  • Equally high efficiency for both histone and other DNA binding protein

Shipping / Storage

Ship and store at room temperature.

Shelf Life

12 months

Manual (protocol)

  101Bio.com
  Histone/DNA Binding Protein Extraction Kit (Cells)

Components

Components Amount Storage
P514-25 P514-50
Buffer A 12 mL 25 mL Room temperature
Buffer B 12 mL 25 mL Room temperature
Protein extraction cartridges 25 50 Room temperature
Collection tubes with cap 25 50 Room temperature

Remarks

  • Read the entire manual carefully. The use of protease inhibitors is optional for this kit. If downstream application takes significant amounts of time or the protein extract will be stored for longer period of time, addition of protease inhibitor to the extract is recommended.
  • For determination of protein concentration, BCA kit (Pierce) is recommended.
  • To study protein phosphorylation, phosphatase inhibitors (such as PhosStop from Roche) should be added to Buffer A prior to use.

High efficient extraction of both Histon and other DNA binding protein

High efficient extraction of both Histon and other DNA binding protein

SDS-PAGE (18%) profiling of extracted histon and DNA binding proteins from different cells.

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