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Nuclear Envelope Protein Extraction Kit  (Cells/tissues)

Name

Nuclear Envelope Protein Extraction Kit (Cells / tissues)

Cat. #

P513L
$499.00
(50 reactions)

Researchers also buy our Protein Analysis Reagents (HRP, Antibody enhancer, WB stripping solution, etc.)
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Application

The kit is developed to rapidly extract native nuclear envelope proteins and its associated proteins from cells or cells isolated from tissues within 45 minutes.

The extracted proteins are native and detergent/EDTA free.

The extracted protein can be used for SDS-PAGE, immunoblottings, ELISA, IP, protein translocation analysis, enzyme activity assays, and other applications.

This kit provides the fastest method currently available for enrichment of native nuclear envelope proteins.

This product is for research use only.

Product Size

P513L: 50 rxn

Description

The nuclear envelope is a very complex membrane-protein system that is notoriously difficult to isolate and purify due to its connection to nuclear and cytoplasmic components. Traditional method of nuclear envelope isolation and purification requires a large amount of starting material and a lengthy and tedious procedure. This kit can extract native nuclear envelope proteins and its associated proteins from cells or cells isolated from tissues in less than 45 minute.

  • Fast - less than 45 minutes
  • High yield and high concentration - 10-50 µg protein/sample
  • Ideal for small amount of cells (only 10 - 20 million cells)

Result

nuclear membrane protein extraction kit

Shipping / Storage

Ship at room temperature. Store at -20℃

Shelf Life

12 months

Manual (protocol)

  101Bio.com
  Nuclear Envelope Protein Extraction Kit (Cells/tissues)

Components

Components Amount Storage
Buffer A 25 mL -20℃
Buffer B 15 mL -20℃
Filter Cartridges 50 Room temperature
Collection tubes with cap 50 Room temperature

Remarks

  • Read the entire procedures carefully. Thaw buffer A and buffer B completely, invert the bottles a few times and place them on ice. Chill protein extraction filter cartridge with collection tube on ice prior to use.
  • All centrifugation steps should be performed at 4oC in a cold room or in a refrigerated mirocentrifuge.
  • For determination of protein concentration, BCA kit (Pierce) is recommended.
  • To study protein phosphorylation, phosphatase inhibitors (such as PhosStop from Roche) should be added to Buffer A prior to use.
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