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Bacteria Total Protein Extraction Kit

Name

3-min. Bacteria Total Protein Extraction kit

Cat. #

P512L
$299.00
(50 reactions)

Researchers also buy our Protein Analysis Reagents (HRP, Antibody enhancer, WB stripping solution, etc.)
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Application

Fast extraction of denatured total proteins from bacteria.

Extracted protein can bed used in SDS-PAGE, immunoblottings, ELISA and other applications.

This product is for research use only.

Product Size

P512L: 50 rxn

Description

This kit provides the most rapid method for preparation of whole bacterial cell extract.

The kit contains sufficient materials for extraction of total proteins from 100 ml E.coli culture.

  • Fast - 3 minutes

Shipping / Storage

Ship and store at room temperature.

Shelf Life

12 months

Manual (protocol)

  101Bio.com
  3-min. Bacteria Total Protein Extraction kit

Components

Components Amount Storage
Bacterial lysis buffer A 25 mL Room temperature
Buffer B 2.5 mL Room temperature
Filter Cartridges 50 Room temperature
Collection tubes with cap 50 Room temperature

Remarks

  • The use of protease inhibitors is not necessary prior to extraction. However if downstream application takes significant amounts of time or the protein extract will be stored for longer period of time, addition of protease inhibitor to extracted lysate is recommended.
  • Multiple filter cartridges may be used if larger amount of cell lysate is desired.
  • To study protein phosphorylation, phosphatase inhibitors must be added to lysis buffer prior to use.
Bacteria Total Protein Extraction Kit
Protein Analysis Reagents
Name Cat. # Size Price Order
101Bio HRP Substrate Kit P5W1 30 ml /30 ml $  99.00
101Bio Antibody Enhancer P5W2 30 ml $  99.00
101Bio WB Stripping Solution P5W3 250 ml $  99.00
101Bio Ponceau S Stain P5W4 250 ml $  39.00
101Bio Peroxidase Suppressor P5W5 30 ml $  60.00
101Bio High Efficiency Protein Precipitation kit P5W6 30 ml /30 ml $129.00
101Bio Red Blood Cell Lysis Buffer P5W7 250 ml $  98.00
101Bio WB Blocking Solution P5W8 250 ml $  99.00
101Bio Denaturing Protein Solubilization Reagent P5W9 10 ml $  30.00
101Bio Non-Denatured Protein Solubilization Reagent P5W10 10 ml $  30.00
101Bio Protein Solubilization Reagent for MS P5W11 2 ml $  30.00
101Bio SDS-Remover P5W12 10 ml $  40.00
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