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Intact Plant Chloroplast Isolation Kit

Name

8-min. Intact Plant Chloroplast Isolation kit

Cat. #

P511L
$299.00
(50 reactions)

Researchers also buy our Protein Analysis Reagents (HRP, Antibody enhancer, WB stripping solution, etc.)
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Application

Fast isolation of intact chloroplasts from fresh plant tissues (leaves, seeds and soft stems etc.)

Yield 1 X 106 to 1 X 107 intact chloroplasts (>90% intact) from 5 ~ 200 mg fresh plant leaves in less than 5 min.

Isolated chloroplasts are ideal starting materials for purification of RNA and DNA, biochemical analysis, SDS-PAGE, immunoblottings, enzyme assays etc.

This product is for research use only.

Product Size

P511L: 50 rxn

Description

This kit contains optimized chloroplast isolation buffers.

Filter cartridges with pre-defined pore size and thickness are used. Intact chloroplasts can be isolated from 5-200 mg fresh plant tissues in less than 5 min.

  • Fast - 5 minutes
  • High yield and high concentration - 1 X 106 to 1 X 107 intact chloroplasts
  • Ideal for small volume of sample - start with 5 ~ 200 mg plant tissue

Shipping / Storage

Ship at room temperature. Store at -20℃.

Shelf Life

12 months

Manual (protocol)

  101Bio.com
  8-min. Intact Plant Chloroplast Isolation kit

Components

Components Amount Storage
Buffer A 25 mL -20℃
Buffer B 25 mL -20℃
Filter Cartridges 50 Room temperature
Collection tubes with cap 50 Room temperature
Plastic rods 4 Room temperature

Remarks

  • Prior to chloroplast isolation Thaw buffer A and buffer B completely and place on ice.
  • This protocol is developed and validated by 101Bio’s OEM partner. Spin column based protein extraction and cell fractionation technologies were developed by 101Bio’s OEM partner.
Intact Plant Chloroplast Isolation Kit
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