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Intact Plant Chloroplast Isolation Kit


8-min. Intact Plant Chloroplast Isolation kit

Cat. #

(50 reactions)

Researchers also buy our Protein Analysis Reagents (HRP, Antibody enhancer, WB stripping solution, etc.)
Also can buy from :


Fast isolation of intact chloroplasts from fresh plant tissues (leaves, seeds and soft stems etc.)

Yield 1 X 106 to 1 X 107 intact chloroplasts (>90% intact) from 5 ~ 200 mg fresh plant leaves in less than 5 min.

Isolated chloroplasts are ideal starting materials for purification of RNA and DNA, biochemical analysis, SDS-PAGE, immunoblottings, enzyme assays etc.

This product is for research use only.

Product Size

P511L: 50 rxn


This kit contains optimized chloroplast isolation buffers.

Filter cartridges with pre-defined pore size and thickness are used. Intact chloroplasts can be isolated from 5-200 mg fresh plant tissues in less than 5 min.

  • Fast - 5 minutes
  • High yield and high concentration - 1 X 106 to 1 X 107 intact chloroplasts
  • Ideal for small volume of sample - start with 5 ~ 200 mg plant tissue

Shipping / Storage

Ship at room temperature. Store at -20℃.

Shelf Life

12 months

Manual (protocol)
  8-min. Intact Plant Chloroplast Isolation kit


Components Amount Storage
Buffer A 25 mL -20℃
Buffer B 25 mL -20℃
Filter Cartridges 50 Room temperature
Collection tubes with cap 50 Room temperature
Plastic rods 4 Room temperature


  • Prior to chloroplast isolation Thaw buffer A and buffer B completely and place on ice.
  • This protocol is developed and validated by 101Bio’s OEM partner. Spin column based protein extraction and cell fractionation technologies were developed by 101Bio’s OEM partner.
Intact Plant Chloroplast Isolation Kit
Protein Analysis Reagents
Name Cat. # Size Price Order
101Bio HRP Substrate Kit P5W1 30 ml /30 ml $  99.00
101Bio Antibody Enhancer P5W2 30 ml $  129.00
101Bio WB Stripping Solution P5W3 250 ml $  129.00
101Bio Ponceau S Stain P5W4 250 ml $  59.00
101Bio Peroxidase Suppressor P5W5 30 ml $  89.00
101Bio High Efficiency Protein Precipitation kit P5W6 30 ml /30 ml $129.00
101Bio Red Blood Cell Lysis Buffer P5W7 250 ml $  109.00
101Bio WB Blocking Solution P5W8 250 ml $  109.00
101Bio Denaturing Protein Solubilization Reagent P5W9 10 ml $  49.00
101Bio Non-Denatured Protein Solubilization Reagent P5W10 10 ml $  49.00
101Bio Protein Solubilization Reagent for MS P5W11 2 ml $  49.00
101Bio SDS-Remover P5W12 10 ml $  79.00
101Bio Albumin Depletion Reagent for Plasma and Serum P5W13 10 ml $  109.00
101Bio Protein A+G Agarose Beads W0349 1 ml $  99.00
101Bio Protease Inhibitor Cocktail (100x) W2200 1 ml $  49.00
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