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Plant Tissue Detergent-free Total Protein Extraction Kit

Name

8-min. Plant tissue Detergent-free Total Protein Extraction Kit

Cat. #

P510L
$299.00
(50 reactions)

Researchers also buy our Protein Analysis Reagents (HRP, Antibody enhancer, WB stripping solution, etc.)
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Application

Extract detergent-free total protein from plant tissues within 8 minutes.

Can handle frozen or fresh leaves, seeds, soft stem and roots, etc.

For proteomics(LC/MS), ELISA, SDS-PAGE, immunoblottings, IP, etc.

This product is for research use only.

Product Size

P510L: 50 rxn

Description

This kit is for fast extraction of water soluble proteins from plant tissues.

The protein extraction does not contain any detergent and organic solvent.

  • Fast - 5 to 8 minutes
  • High yield and high concentration - 1 ~ 5 mg/ml
  • Ideal for small volume of sample - extraction volume can be 50 ~ 500 uL

Shipping / Storage

Ship at room temperature. Store at 4℃. (Do not freeze.)

Shelf Life

12 months

Manual (protocol)

  101Bio.com
  8-min Plant Tissue Detergent-free Total Protein Extraction Kit

Components

Components Amount Storage
Detergent-free lysis buffer 25 mL 4℃
Filter Cartridges 50 Room temperature
Collection tubes with cap 50 Room temperature
Plastic rods 4 Room temperature

Remarks

  • Total proteins can be extracted from plant tissue in 5-8 min
  • Protease inhibitors is not necessary prior to extraction. However if downstream application takes significant amounts of time or the protein extract will be stored for longer period of time, addition of protease inhibitors to lysis buffer is recommended.
  • For determination of protein concentration, BCA kit (Pierce) is recommended.
  • To study protein phosphorylation, phosphatase inhibitors (such as PhosStop from Roche) should be added to lysis buffer prior to use.
Protein Analysis Reagents
Name Cat. # Size Price Order
101Bio HRP Substrate Kit P5W1 30 ml /30 ml $  99.00
101Bio Antibody Enhancer P5W2 30 ml $  99.00
101Bio WB Stripping Solution P5W3 250 ml $  99.00
101Bio Ponceau S Stain P5W4 250 ml $  39.00
101Bio Peroxidase Suppressor P5W5 30 ml $  60.00
101Bio High Efficiency Protein Precipitation kit P5W6 30 ml /30 ml $129.00
101Bio Red Blood Cell Lysis Buffer P5W7 250 ml $  98.00
101Bio WB Blocking Solution P5W8 250 ml $  99.00
101Bio Denaturing Protein Solubilization Reagent P5W9 10 ml $  30.00
101Bio Non-Denatured Protein Solubilization Reagent P5W10 10 ml $  30.00
101Bio Protein Solubilization Reagent for MS P5W11 2 ml $  30.00
101Bio SDS-Remover P5W12 10 ml $  40.00
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