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Plant Tissue Total Protein Extraction Kit

Name

8-min. Plant Tissue Total Protein Extraction Kit

Cat. #

P508L
$299.00
(50 reactions)
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Application

Extract plan tissue total protein in < 8 minute.

Can handle frozen or fresh leaves, seeds, soft stem and roots, etc.

Extract native or denatured protein.

For SDS-PAGE, immunoblottings, ELISA, IP, enzyme assays etc.

Extracted proteins can be use as a good starting material for small scale protein purification in column chromatography.

This product is for research use only.

Product Size

P508L: 50 rxn

Description

This kit is for fast extraction of total proteins from plant tissues.

  • Fast - 5 to 8 minutes
  • High yield and high concentration - 2 ~ 8 mg/ml
  • Ideal for small volume of sample - can handle 20 ~ 200 mg plant tissue.

Shipping / Storage

Ship at room temperature. Store "Native lysis buffer" at 4℃, and the rest at room temperature. (Do not freeze.)

Shelf Life

12 months

Manual (protocol)

  101Bio.com
  8-min. Plant tissue Total Protein Extraction Kit

Citations

Citation 1:
Xianjun Peng, Qi Wang, Hui Liu,Shihua Shen. Phylogenetic and functional analysis of the basic transcription factor gene BTF3 from Jatropha curcas. Plant Growth Regul. June 2017, Volume 82, Issue 2, pp 247–257.

Components

Components Amount Storage
Denaturing lysis buffer 25 mL Room temperature
Native lysis buffer 25 mL Room temperature
Filter Cartridges 50 Room temperature
Collection tubes with cap 50 Room temperature
Plastic rods 5 Room temperature

Remarks

  • Protein profiles extracted by denaturing and native lysis buffer are not identical. For a given application one lysis buffer might be superior to the other. This kit provides both denaturing and native cell lysis buffers for users to test and select the best one for a specific application.
  • Total proteins can be extracted from plant tissue in 5-8 min
  • Protease inhibitors is not necessary prior to extraction. However if downstream application takes significant amounts of time or the protein extract will be stored for longer period of time, addition of protease inhibitors to lysis buffer is recommended.
  • For determination of protein concentration, BCA kit (Pierce) is recommended.
  • To study protein phosphorylation, phosphatase inhibitors (such as PhosStop from Roche) should be added to lysis buffer prior to use.
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