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Mitochondria Isolation Kit  (Mammalian cells / tissues)

Name

Mitochondria Isolation Kit (Mammalian cells / tissues)

Cat. #

P507L
$399.00
(50 reactions)

Researchers also buy our Protein Analysis Reagents (HRP, Antibody enhancer, WB stripping solution, etc.)
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Application

Rapidly isolate intact mitochondria from mammalian cells or tissues in less than 30 minute.

This product is for research use only.

Product Size

P507L: 50 rxn

Description

This kit is for fast isolation of intact mitochondria from animal tissues. The isolated mitochondria does not contain any detergent and EDTA.

  • Fast - less than 30 minutes
  • High yield and high concentration - 1 ~ 3 mg/ml
  • Ideal for small volume of sample - can handle 5 - 40 million cells

Shipping / Storage

Ship at room temperature. Store at -20℃

Shelf Life

12 months

Manual (protocol)

  101Bio.com
  Mitochondria Isolation Kit (Mammalian cells / tissues)

Components

Components Amount Storage
Buffer A 15 mL -20℃
Buffer B 30 mL -20℃
Filter Cartridges 50 Room temperature
Collection tubes with cap 50 Room temperature
Plastic rods 4 Room temperature
Tissue dissociation beads 4g Room temperature

Remarks

  • Protease inhibitors is not necessary prior to extraction. However if downstream application takes significant amounts of time or the protein extract will be stored for longer period of time, addition of protease inhibitors to Buffer A is recommended.
  • For determination of protein concentration, BCA kit (Pierce) is recommended.
  • To study protein phosphorylation, phosphatase inhibitors (such as PhosStop from Roche) should be added to Buffer A prior to use.
  • This protocol is developed and validated by 101Bio’s OEM partner. Spin column based protein extraction and cell fractionation technologies were developed by 101Bio’s OEM partner.
Protein Analysis Reagents
Name Cat. # Size Price Order
101Bio HRP Substrate Kit P5W1 30 ml /30 ml $  99.00
101Bio Antibody Enhancer P5W2 30 ml $  99.00
101Bio WB Stripping Solution P5W3 250 ml $  99.00
101Bio Ponceau S Stain P5W4 250 ml $  39.00
101Bio Peroxidase Suppressor P5W5 30 ml $  60.00
101Bio High Efficiency Protein Precipitation kit P5W6 30 ml /30 ml $129.00
101Bio Red Blood Cell Lysis Buffer P5W7 250 ml $  98.00
101Bio WB Blocking Solution P5W8 250 ml $  99.00
101Bio Denaturing Protein Solubilization Reagent P5W9 10 ml $  30.00
101Bio Non-Denatured Protein Solubilization Reagent P5W10 10 ml $  30.00
101Bio Protein Solubilization Reagent for MS P5W11 2 ml $  30.00
101Bio SDS-Remover P5W12 10 ml $  40.00
101Bio Protein A+G Agarose Beads W0349 1 ml $  99.00
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