Also available at    &     VWR
Detergent-free Total Protein Extraction Kit     (Animal cells)

Name

Detergent-free Total Protein Extraction Kit (Animal cells)

Cat. #

P505S
$179.00
(  5 reactions)

P505
$279.00
(20 reactions)

P505L
$419.00
(50 reactions)

Researchers also buy our Protein Analysis Reagents (HRP, Antibody enhancer, WB stripping solution, etc.)
Also can buy from :

Application

Rapidly extract detergent-free total proteins from cultured cells (mammalian cells, insect cells and other cultured cells) in less than 5 minute.

The extracted protein can be used for proteomics (LC/MS), IP, ELISA, 2D-gel analysis, isoelectric focusing, SDS-PAGE, immunoblottings, and other applications.

This product is for research use only.

Description

This kit is for fast extraction of detergent-free total proteins from animal cells. The extraction does not contain any detergent and EDTA. The extraction volume is flexible, from 20 µl to 500 µl, which is useful for limiting number of cells.

  • Fast - less than 5 minutes
  • High yield and high concentration - 1 ~ 5 mg/ml
  • Ideal for small volume of sample - can handle 0.5 ~ 3 million cells

Shipping / Storage

Ship at room temperature. Store at 4℃ (Do not freeze.)

Shelf Life

12 months

Manual (protocol)

  101Bio.com
  Detergent-free Total Protein Extraction Kit (Animal cells)

Components

Components Amount Storage
Buffer A 6 mL 4℃
Buffer B 6 mL 4℃
Filter Cartridges 20 Room temperature
Collection tubes with cap 20 Room temperature

Remarks

  • Detergent-free total proteins can be extracted from cells in less than 5 minutes.
  • Protease inhibitors is not necessary prior to extraction. However if downstream application takes significant amounts of time or the protein extract will be stored for longer period of time, addition of protease inhibitors to Buffer A is recommended.
  • For determination of protein concentration, BCA kit (Pierce) is recommended.
  • To study protein phosphorylation, phosphatase inhibitors (such as PhosStop from Roche) should be added to Buffer A prior to use.
  • This protocol is developed and validated by 101Bio’s OEM partner. Spin column based protein extraction and cell fractionation technologies were developed by 101Bio’s OEM partner.
Detergent-free Total Protein Extraction Kit for Animal cells
Protein Analysis Reagents
Name Cat. # Size Price Order
101Bio HRP Substrate Kit P5W1 30 ml /30 ml $  99.00
101Bio Antibody Enhancer P5W2 30 ml $  99.00
101Bio WB Stripping Solution P5W3 250 ml $  99.00
101Bio Ponceau S Stain P5W4 250 ml $  39.00
101Bio Peroxidase Suppressor P5W5 30 ml $  60.00
101Bio High Efficiency Protein Precipitation kit P5W6 30 ml /30 ml $129.00
101Bio Red Blood Cell Lysis Buffer P5W7 250 ml $  98.00
101Bio WB Blocking Solution P5W8 250 ml $  99.00
101Bio Denaturing Protein Solubilization Reagent P5W9 10 ml $  30.00
101Bio Non-Denatured Protein Solubilization Reagent P5W10 10 ml $  30.00
101Bio Protein Solubilization Reagent for MS P5W11 2 ml $  30.00
101Bio SDS-Remover P5W12 10 ml $  40.00
101Bio Albumin Depletion Reagent for Plasma and Serum P5W13 10 ml $  99.00
101Bio Protein A+G Agarose Beads W0349 1 ml $  99.00
101Bio Protease Inhibitor Cocktail (100x) W2200 1 ml $  49.00
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