Also available at    &     VWR
Cytoplasmic & Nuclear Protein Extraction Kit     (cells & tissues)


8-min. Cytoplasmic & Nuclear Protein Extraction Kit (cells & tissues)

Cat. #

(  4 reactions)    contact Fisher

(20 reactions)    order from  Fisher
(Fisher Cat.# NC1337256)

(50 reactions)    contact Fisher

Researchers also buy our Protein Analysis Reagents (HRP, Antibody enhancer, WB stripping solution, etc.)
Also can buy from :


Fastest method to extract native cytoplasmic and nuclear protein from mammalian cells or tissues.

Total procedure is less than 10 minutes.

Extracted protein can be used for SDS-PAGE, immunoblottings, ELISA, IP, protein localization, gel mobility shift assays, 2-D gels, etc.

This product is for research use only.


This kit is for fast extraction of native cytoplasmic and nuclear proteins from cultured mammalian cells or tissues.

This product works as good as the one sold by Thermo Fisher (Cat.# 78833) but 9 times faster (8 min. vs. 70 min.)

  • Fast - as quick as 5 to 8 minutes
  • 50% higher nuclear protein yield than other brand kits

Shipping / Storage

Ship at room temperature. Store at 4℃

Shelf Life

12 months

Manual (protocol)
  Cytoplasmic & Nuclear Protein Extraction Kit (cells & tissues)


Citation 1:
Frismantiene A, Dasen B, Pfaff D, Erne P, Resink TJ, Philippova M. T-cadherin promotes vascular smooth muscle cell dedifferentiation via a GSK3β-inactivation dependent mechanism. Cell Signal. 2016 Feb 22;28(5):516-530. doi: 10.1016/j.cellsig.2016.02.014.   
Citation 2:
Matsumura K, Nakazawa T, Nagayasu K, Gotoda-Nishimura N, Kasai A, Hayata-Takano A, Shintani N, Yamamori H, Yasuda Y, Hashimoto R, Hashimoto H. De novo POGZ mutations in sporadic autism disrupt the DNA-binding activity of POGZ. J Mol Psychiatry. 2016 Apr 21;4:1. doi: 10.1186/s40303-016-0016-x. eCollection 2016.
Citation 3:
Koichi Inouea, Eisuke Sakuma, Hiroyuki Morimoto, Hayato Asai, Yoshinori Koide, Tiandong Leng, Ikuo Wada, Zhi-Gang Xiong, Takatoshi Ueki. Serum- and glucocorticoid-inducible kinases in microglia. Biochem Biophys Res Commun. 2016 Sep 9;478(1):53-9. doi: 10.1016/j.bbrc.2016.07.094. Epub 2016 Jul 22.


Components Amount Storage
Cat.#: P504S Cat.#: P504 Cat.#: P504L
Cytoplamic extraction buffer 2 mL 10 mL 25 mL 4℃
Nuclear extraction buffer 2 mL 10 mL 25 mL 4℃
Filter Cartridges 4 20 50 Room temperature
Collection tubes with cap 4 20 50 Room temperature


  • Protease inhibitors is not necessary prior to extraction. However if downstream application takes significant amounts of time or the protein extract will be stored for longer period of time, addition of protease inhibitors to extracted lysate buffer is recommended.
  • The nuclear extraction buffer contains 300 mM salt, for some applications, dilution or desalting of the extract may be needed.
  • The capacity of protein extraction filter cartridge is 500 µL. Multiple filter cartridges can be used if larger amount of cell lysate is processed.
  • For determination of protein concentration, BCA kit (Pierce) is recommended.
  • To study protein phosphorylation, phosphatase inhibitors (such as PhosStop from Roche) should be added to lysis buffer prior to use.
  • This protocol is developed and validated by 101Bio’s OEM partner. Spin column based protein extraction and cell fractionation technologies were developed by 101Bio’s OEM partner.
Cytoplasmic and nuclear protein extraction kit
Protein Analysis Reagents
Name Cat. # Size Price Order
101Bio HRP Substrate Kit P5W1 30 ml /30 ml $  99.00
101Bio Antibody Enhancer P5W2 30 ml $  129.00
101Bio WB Stripping Solution P5W3 250 ml $  129.00
101Bio Ponceau S Stain P5W4 250 ml $  59.00
101Bio Peroxidase Suppressor P5W5 30 ml $  89.00
101Bio High Efficiency Protein Precipitation kit P5W6 30 ml /30 ml $129.00
101Bio Red Blood Cell Lysis Buffer P5W7 250 ml $  109.00
101Bio WB Blocking Solution P5W8 250 ml $  109.00
101Bio Denaturing Protein Solubilization Reagent P5W9 10 ml $  49.00
101Bio Non-Denatured Protein Solubilization Reagent P5W10 10 ml $  49.00
101Bio Protein Solubilization Reagent for MS P5W11 2 ml $  49.00
101Bio SDS-Remover P5W12 10 ml $  79.00
101Bio Albumin Depletion Reagent for Plasma and Serum P5W13 10 ml $  109.00
101Bio Protein A+G Agarose Beads W0349 1 ml $  99.00
101Bio Protease Inhibitor Cocktail (100x) W2200 1 ml $  49.00
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