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Plasma Membrane Protein Extraction Kit  (mammalian cells or tissues)

Name

Plasma Membrane Protein Extraction Kit   (mammalian cells or tissues)

Cat. #

P503S
$119.00
(  5 reactions)    order from  Fisher (Fisher Cat.# NC1053481)

P503
$249.00
(20 reactions)    order from  Fisher (Fisher Cat.# NC1053480)

P503L
$419.00
(50 reactions)    order from  Fisher (Fisher Cat.# NC1053482)

Researchers also buy our Protein Analysis Reagents (HRP, Antibody enhancer, WB stripping solution, etc.)
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Application

From mammalian cells or tissues, rapid extract 4 fractions within 45 minutes:
  • Plasma membrane protein
  • Organelle membrane proteins
  • Cytosol protein
  • Nuclear protein
  • Native and EDTA free

This product is for research use only.

Description

This Plasma Membrane Protein Extraction Kit is designed to rapidly isolate native total membrane proteins (organelle membrane proteins) and native plasma membrane proteins from cultured mammalian cells or tissues.

  • Simple and user friendly
  • Fast: 45 minutes
  • Wide range of starting cells (1 - 50 millions / sample)
  • Detergent and EDTA free
  • No need for Dounce homogenizer or tissue blender
  • The procedure can be completed in less than 45 min.
  • High yield

This kit is composed of optimized buffers (detergent and EDTA free) and protein extraction filter cartridges with 2.0 ml collection tubes.

Result

plasma membrane protein extraction kit

Shipping / Storage

Ship at 4℃. Store Buffer A and Buffer B at -20℃ upon arrival, and the rest at room temperature.

Shelf Life

12 months

Manual (protocol)

  101Bio.com
  Plasma Membrane Protein Extraction Kit Manual

Citations

Citation 1:
Shibata T, Takahashi K, Matsubara Y, Inuzuka E, Nakashima F, Takahashi N, Kozai D, Mori Y, Uchida K. Identification of a prostaglandin D2 metabolite as a neuritogenesis enhancer targeting the TRPV1 ion channel. Sci Rep. 2016 Feb 16;6:21261. doi: 10.1038/srep21261.   
Citation 2:
Ramsey J, Renzi EC, Arnold RJ, Trinidad JC, Mukhopadhyay S. Palmitoylation of Sindbis Virus TF Protein Regulates Its Plasma Membrane Localization and Subsequent Incorporation into Virions. J Virol. 2017 Jan 18;91(3). pii: e02000-16. doi: 10.1128/JVI.02000-16. Print 2017 Feb 1.   
Citation 3:
Ishigaki H, Maeda T, Inoue H, Akagi T, Sasamura T, Ishida H, Inubushi T, Okahara J, Shiina T, Nakayama M, Itoh Y, Ogasawara K. Transplantation of iPS-Derived Tumor Cells with a Homozygous MHC Haplotype Induces GRP94 Antibody Production in MHC-Matched Macaques. Cancer Res. 2017 Nov 1;77(21):6001-6010. doi: 10.1158/0008-5472.CAN-17-0775. Epub 2017 Sep 7.    

Components

Components Amount Storage
Cat.#: P503S Cat.#: P503   Cat.#: P503L
Buffer A 2.5 mL 10 mL 25 mL -20℃
Buffer B 1 mL 4 mL 10 mL -20℃
Protein Extraction Filter Cartridges 5 20 50 Room temperature
Collection tubes with cap 5 20 50 Room temperature
Plastic rods 1 1 4 Room temperature
Tissue dissociation beads 0.5g 2g 5g Room temperature

Remarks

  • Protease inhibitors is not necessary prior to extraction. However if downstream application takes significant amounts of time or the protein extract will be stored for longer period of time, addition of protease inhibitors to cell lysis buffer is recommended.
  • For determination of protein concentration, BCA kit (Pierce) is recommended.
  • To study protein phosphorylation, phosphatase inhibitors (such as PhosStop from Roche) should be added to lysis buffer prior to use.
  • This protocol is developed and validated by 101Bio’s OEM partner. Spin column based protein extraction and cell fractionation technologies were developed by 101Bio’s OEM partner.
Protein Analysis Reagents
Name Cat. # Size Price Order
101Bio HRP Substrate Kit P5W1 30 ml /30 ml $  99.00
101Bio Antibody Enhancer P5W2 30 ml $  99.00
101Bio WB Stripping Solution P5W3 250 ml $  99.00
101Bio Ponceau S Stain P5W4 250 ml $  39.00
101Bio Peroxidase Suppressor P5W5 30 ml $  60.00
101Bio High Efficiency Protein Precipitation kit P5W6 30 ml /30 ml $129.00
101Bio Red Blood Cell Lysis Buffer P5W7 250 ml $  98.00
101Bio WB Blocking Solution P5W8 250 ml $  99.00
101Bio Denaturing Protein Solubilization Reagent P5W9 10 ml $  30.00
101Bio Non-Denatured Protein Solubilization Reagent P5W10 10 ml $  30.00
101Bio Protein Solubilization Reagent for MS P5W11 2 ml $  30.00
101Bio SDS-Remover P5W12 10 ml $  40.00
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54.162.227.37