Also available at    &     VWR
Plasma Membrane Protein Isolation Kit for Plants

Name

Plasma Membrane Protein Isolation Kit for Plants

Cat. #

P503P-25
$319.00
(25 reactions)

P503P-50
$599.00
(50 reactions)

Researchers also buy our Protein Analysis Reagents (HRP, Antibody enhancer, WB stripping solution, etc.)
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Application

The kit is designed to rapidly isolate native membrane proteins from plant tissues for applications such as SDS-PAGE, immunoblottings, ELISA, IP, membrane protein structure analysis, 2-D gels, enzyme activity assays and other applications.

The procedure can be completed in about 1 hour.

This product is for research use only.

Description

Plasma membrane (PM) protein accounts for a small fraction of total cellular protein in plants but performs a very critical role in plant physiology. Isolation and purification of PM protein from plant tissues have been traditionally done by sucrose density ultracentrifugation and aqueous two-phase partitioning. These methods, while relatively effective, require ultracentrifugation and large amount of starting material. The procedures are usually tedious and time consuming. To overcome the shortcomings, we have developed this PM isolation kit. Plant tissues are first sensitized by Buffer A, homogenized, and pass through a specialized filter cartridge that allows homogenates to pass through a zigzag path. The cell membranes are ruptured into a range of predefined size during the process. Native plasma membranes are separated from a mixture of un-ruptured cells, nuclei, cytosol and organelles by subsequent differential centrifugation and density centrifugation without using ultracentrifugation. Due to the use of the same amount of starting material, defined centrifugal force and pre-defined duration in every experiment, the result is much more consistent with higher degree of PM protein enrichment than ultracentrifugation. The procedure can be completed in about 1 hour.

  • Easy to use
  • Fast: 1 hour
  • Native protein
  • Detergent and EDTA free
  • High yield

Shipping / Storage

Ship at RT. Store Buffer A and Buffer B at -20℃ upon arrival, and the rest at RT.

Shelf Life

12 months

Manual (protocol)

  101Bio.com
  Plasma Membrane Protein Isolation Kit for Plants Manual

Components

Components Amount Storage
Cat.# P503P-25 P503P-50
Buffer A 12.5 mL 25 mL -20℃
Buffer B 5 mL 10 mL -20℃
Protein Extraction Filter Cartridges 25 50 Room temperature
Collection tubes with cap 25 50 Room temperature
Plastic rods 2 4 Room temperature
Tissue dissociation beads 2.5g 5g Room temperature

Remarks

  • All centrifugation steps should be performed at 4oC (either in a cold room or in a refrigerated microcentrifuge).
  • To study protein phosphorylation, phosphatase inhibitors (such as PhosStop from Roche) should be added to Buffer A prior to use. The use of protease inhibitor cocktails is optional.
  • It is recommended to use BCA Protein Assay Kit for determination of protein concentration.
  • The rpm in this protocol is based on A Eppendoff 5415C table top microcentrifuge.
  • This protocol is developed and validated by 101Bio’s OEM partner. Spin column based protein extraction and cell fractionation technologies were developed by 101Bio’s OEM partner.
Protein Analysis Reagents
Name Cat. # Size Price Order
101Bio HRP Substrate Kit P5W1 30 ml /30 ml $  99.00
101Bio Antibody Enhancer P5W2 30 ml $  99.00
101Bio WB Stripping Solution P5W3 250 ml $  99.00
101Bio Ponceau S Stain P5W4 250 ml $  39.00
101Bio Peroxidase Suppressor P5W5 30 ml $  60.00
101Bio High Efficiency Protein Precipitation kit P5W6 30 ml /30 ml $129.00
101Bio Red Blood Cell Lysis Buffer P5W7 250 ml $  98.00
101Bio WB Blocking Solution P5W8 250 ml $  99.00
101Bio Denaturing Protein Solubilization Reagent P5W9 10 ml $  30.00
101Bio Non-Denatured Protein Solubilization Reagent P5W10 10 ml $  30.00
101Bio Protein Solubilization Reagent for MS P5W11 2 ml $  30.00
101Bio SDS-Remover P5W12 10 ml $  40.00
101Bio Albumin Depletion Reagent for Plasma and Serum P5W13 10 ml $  99.00
101Bio Protein A+G Agarose Beads W0349 1 ml $  99.00
101Bio Protease Inhibitor Cocktail (100x) W2200 1 ml $  49.00
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