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  Total Protein Extraction Kit  (Animal Cells)
Warning:   Using RIPA buffer, you get biased data (Western Blot, IP, ELISA, etc.) due to uneven protein loss.  
Replace it by our P501 which can extract full spectrum total protein in 3 minutes.

Name

3-min. Total Protein Extraction Kit (Animal Cells)

Cat. #

P501S
$  99.00
(  5 reactions)    contact Fisher

P501
$179.00
(20 reactions)    order from  Fisher
(Fisher Cat.# NC1337255)

P501L
$279.00
(50 reactions)    contact Fisher
Also can buy from :

Application

Extract full spectrum total protein in 3 minutes. This product is for research use only.

Description

RIPA buffer has been used as a “gold standard” for total protein extraction for 20 years. In fact, it fails to extract true total protein. Due to its method defect, RIPA produce biased data (Western Blot, IP, ELISA, etc.) caused by uneven protein loss and leads to wrong interpretations. Recently, it has been reported by more and more researchers that the data from RIPA are questionable.

Our P501 is an ultimate solution which can extract ture total protein (full spectrum protein profile).

The total protein extracted by our P501 can be used in all Western Blot, IP and co-IP assay.

  • Fast - 1 to 3 minutes
  • Full spectrum protein profile
  • For all animal cells: mammalian, insets and other cells
  • Flexible extraction volume: 20 µl to 500 µl (useful for limiting number of cells)
  • High yield and high concentration - 2 ~ 8 mg/ml
  • Ideal for small volume of sample - can handle 0.3 ~ 3 million cells

Shipping / Storage

Ship at room temperature. Store "Native cell lysis buffer" at 4℃, and the rest at room temperature. (Do not freeze.)

Shelf Life

12 months

Manual (protocol)

  101Bio.com
  Total Protein Extraction Kit (Animal Cells)

Components

Components Amount Storage
Denaturing cell lysis buffer 10 mL Room temperature
Native cell lysis buffer 10 mL 4℃
Filter Cartridges 20 Room temperature
Collection tubes with cap 20 Room temperature

Remarks

  • Protein profiles extracted by denaturing and native cell lysis buffer are not identical. For a given application one lysis buffer might be superior to the other. This kit provides both denaturing and native cell lysis buffers for users to test and select the best one for a specific application.
  • Total proteins can be extracted from cells in 1-8 min
  • Protease inhibitors is not necessary prior to extraction. However if downstream application takes significant amounts of time or the protein extract will be stored for longer period of time, addition of protease inhibitors to cell lysis buffer is recommended.
  • For determination of protein concentration, BCA kit is recommended.
  • To study protein phosphorylation, phosphatase inhibitors (such as PhosStop from Roche) should be added to lysis buffer prior to use.
total protein extraction kit, animal cells, protein purification

total protein extraction kit,animal cells, protein purification

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