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ExoFectin® sRNA-into-Exosome Kit (Chemical)

Name

ExoFectin® sRNA-into-Exosome Kit (Chemical)

Cat. #

P401
$279.00
(20 reactions)

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Application

ExoFectin® sRNA-into-Exosome Kit (Chemical) is for the loading of nucleic acids including miRNAs and siRNA into pure exosomes isolated by our kits. This product is for research use only.

Product Size

100 μl ExoFectin Reagent (20 reactions)

Yield

30% ~ 65% transfection efficiency

Description

ExoFectin® sRNA-into-Exosome Kit (chemical) is a unique blend of polymers designed for the delivery of small RNAs including miRNA and siRNA into exosomes. This transfection reagent provides highly efficient loading abilities of miRNA or siRNA into exosomes, allowing modified exosomes to carry and deliver small RNAs into target cells. This kit provides the following advantages:

  • efficient gene delivery
  • gentle on treated exosomes
  • no small RNA aggregation in exosome

Shipping / Storage

Ship and store at 4℃. (Do not freeze.)

Shelf Life

6 months

Manual (protocol)

Citation

Citation 1:
Salama A, Fichou N, Allard M, Dubreil L, De Beaurepaire L, Viel A, Jégou D, Bösch S, Bach JM. MicroRNA-29b modulates innate and antigen-specific immune responses in mouse models of autoimmunity. PLoS One. 2014 Sep 9;9(9):e106153. doi: 10.1371/journal.pone.0106153.  (Sold through our European distributor Gentaur.)

Components

 Components Amount Storage
ExoFectin Reagent 100 μl 4℃
Supplemental buffer 1000 μl 4℃

Remarks

  • The efficiency of transfection depends on the quality of the exosomes. We do not suggest to start with impure exosome samples prepared by PEG method.
  • The efficiency of transfection also depends on the optimal concentration of the exosomes and small RNA. Different RNA and exosome from different source may have different optimal concentration. We suggest customer to test different combinations in the first experiment.
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