Also available at    &     VWR

Detergent-free Exosomal Protein Extraction Kit for proteomics (LC/MS)

Name

Detergent-free Exosomal Protein Extraction Kit for proteomics (LC/MS)

Cat. #

P201
$369.00  

P201S
$  99.00  

Also can buy from :

Application

For extracting detergent-free exosomal protein from pure exosome isolated by our Exosome Isolation Kits (Cat.#: P100, P101, P120, P121). This product is for research use only.

Product Size

P201: 10 reactions; P201S: 2 reactions

Yield

In each reaction, 150 ~ 200 µg exosomal protein can be extracted from 10 µl ~ 50 µL pure exosome.

Description

This kit is for rapidly extract detergent-free total proteins from exosome in less than 5 minute. The protein extraction buffers do not contain any detergent and EDTA. The whole procedure is less than 5 minutes.
The extracted protein can be used for proteomics (LC/MS), IP, ELISA, 2D-gel analysis, isoelectric focusing, SDS-PAGE, immunoblottings, and other applications.

Shipping / Storage

Ship room temperature and store at 4℃.

Shelf Life

12 months

Manual (protocol)

  101Bio.com
   Detergent-free Exosomal Protein Extraction Kit

Components

Components P201 P201S Storage
Buffer A 500 µl 100 µl 4℃
Buffer B 500 µl 100 µl 4℃

Remarks

The use of protease inhibitors is not necessary prior to extraction. However if downstream application takes significant amounts of time or the protein extract will be stored for longer period of time, addition of protease inhibitors to Buffer A is recommended. For determination of protein concentration, BCA kit is recommended. To study protein phosphorylation, phosphatase inhibitors (such as PhosStop from Roche) should be added to Buffer A prior to use.
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