Also available at    &     VWR

Exosomal RNA and Protein Extraction kit (Cat.#: P200, P200R, P200P)

Name

Exosomal RNA and Protein Extraction Kit (Cat.# P200)

Cat. #

P200
$649.00
order from  Fisher   (Fisher Cat.# NC0874918)

P200R
$369.00
(Exosomal RNA Extraction Kit only)

P200P
$369.00
(Exosomal Protein Lysis Buffer only)

Also can buy from :

Application

For extracting both exosomal RNA (including microRNA) and/or exosomal protein from pure exosome isolated by our Exosome Isolation Kits (Cat.#: P100, P101, P120, P121). This product is for research use only.

Product Size

20 reactions

Yield

In each reaction, from 50 µL ~ 100 µL pure exosome, 50 ~ 100 ng exosomal RNA or 150 ~ 200 µg exosomal protein can be extracted.

Shipping / Storage

Ship at 4℃, and store at -20℃ and room temperature (see component stable below). Upon receiving keep all bottles upright, at proper temperature (component table below) in dark place.

Shelf Life

6 months

Manual (protocol)

  101Bio.com
   Cat.# P200     Exosomal RNA and Protein Extraction Kit


  101Bio.com
   Cat.# P200R   Exosomal RNA Extraction Kit


  101Bio.com
   Cat.# P200P   Exosomal Protein Lysis Buffer

Citation

Citation 1:
Li X, Liu L, Yang J, Yu Y, Chai J, Wang L, Ma L, Yin H. Exosome Derived From Human Umbilical Cord Mesenchymal Stem Cell Mediates MiR-181c Attenuating Burn-induced Excessive Inflammation. EBioMedicine. 2016, http://dx.doi.org/10.1016/j.ebiom.2016.04.030

Components

Components (Cat.# P200R) Amount Storage
N1 5 mL Room temperature
N2 1 mL Room temperature
N3 2.5 mL Room temperature
N4 10 mL Room temperature
RNA Elution Buffer 0.5 mL Room temperature

Components (Cat.# P200P) Amount Storage
Exosomal Protein Lysis Buffer * 2 mL - 20℃

* Store at 4℃ for 7 days or aliquot and store at - 20℃ for up to 6 months.

Remarks

Important: RNA is sensitive to RNase. Before starting RNA extraction, prepare clean lab bench and wipe working surface and pipettors with RNase decontamination solution, such as Ambion® RNaseZap®. Always wear clean laboratory gloves during manipulation.
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