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PureExo® Exosome Isolation Kit (for serum or plasma)    
Why our exosome so pure?
Answer: By targeted filtration, our filter of specific binding treatment removes protein contaminant to isolate exosome at 95% high purity.        Click to see pure exosome images

Name

PureExo® Exosome Isolation Kit (for serum or plasma)

Cat. #

P101S
$  109.00
(  2 reactions)    contact Fisher

P101
$  299.00
(10 reactions)    order from  Fisher                               (Fisher Cat.# NC0874905)

P101L
$1149.00
(40 reactions)    contact Fisher

P101H
$  129.00
(P101 Sample Buffer, 20 ml)

P101K
$  269.00
(P101 Solution A, B and C, 10 rxn)

P101NGS
$  799.00
(Serum/Plasma→Exosome→RNA→NGS)

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<#1 Exosome Isolation Kit
Exosome vs endosome

Application

Isolate pure exosome from serum and plasma. This kit yields much higher purity than exosome precipitation method. This product is for research use only.

Yield

Each reaction can process 100 ~ 500 µl serum/ plasma. Each reaction can yield pure exosome suspended in 100 ~ 200 µl PBS (from which 300 ~ 400 µg exosomal protein or 200 ~ 300 ng exosomal RNA can be extracted using our Exosomal RNA and Protein Extraction Kit, Cat.#: P200).

Description

P101 kit is for exosome purification / isolation at high yield and purity from serum or plasma.

  • Easy to use: No ultra-centrifugation (< 2 hours)
  • 10-fold higher yield (vs. other kits and ultracentrifuge)
  • Save cost (vs. antibody-bead method)
  • Isolate Pure exosome (95% purity vs. 25% by exosome precipitation method)
  • Intact exosome (good morphology)

Use as little as 100 µl serum to achieve high yield of exosomes for any downstream applications: EM study, exosome label, exosome subpopulation, qRT-PCR profiling of exosomal miRNAs, and gel analysis of exosomal proteins.

Shipping / Storage

Ship and store at room temperature. Upon receiving keep all bottles upright, in cool and dark place.

Shelf Life

12 months

Updated Version Manual (protocol)

exosome concentration, exosome concentration method, how to purify exosome
101Bio.com     Exosome Isolation Kit

Serum and plasma preparation

101Bio.com     Exosome Isolation Kit

Citation

Citation 1:
Chen L, Chen R, Kemper S, Charrier A, Brigstock DR. Suppression of fibrogenic signaling in hepatic stellate cells by Twist1-dependent microRNA-214 expression: Role of exosomes in horizontal transfer of Twist1. Am J Physiol Gastrointest Liver Physiol. 2015 Sep 15;309(6):G491-9. doi: 10.1152/ajpgi.00140.2015. Epub 2015 Jul 30.  
This paper is awarded TOP 10 best original research papers published by the American Physiological Society (APS Select). Link
(APSselect is a collection of the very best original research papers published by the American Physiological Society. Each month, the most outstanding recently published papers from our ten research journals are selected and made available through this multi-journal website.)
Citation 2:
Osteikoetxea X, Németh A, Sódar BW, Vukman KV, Buzás EI. Extracellular vesicles in cardiovascular diseases, are they Jedi or Sith?
J Physiol. 2016 Feb 13. doi: 10.1113/JP271336   
Citation 3:
William G. Whitford    Exosomes: A Major Phenomenon Flying Under the Radar cellculturedish.com   2015/09
Citation 4:
Chen L., Chen R., Brigstock D. MicroRNA profiling of circulating exosomes during experimental liver fibrosis. Hepatology. AASLD abstract, 2014 Oct. (Presidential Honored Distinct Abstract).  
Citation 5:
Li Chen and David R. Brigstock. Cellular or Exosomal microRNAs Associated with CCN Gene Expression in Liver Fibrosis. CCN Proteins. Volume 1489 of the series Methods in Molecular Biology pp 465-480.  
Citation 6:
Kayo Nakamura, Masatoshi Jinnin*, Miho Harada, Hideo Kudo, Wakana Nakayama, Kuniko Inoue, Aki Ogata, Ikko Kajihara, Satoshi Fukushima, Hironobu Ihn. Altered expression of CD63 and exosomes in scleroderma dermal fibroblasts. J Dermatol Sci.2016 Oct;84(1):30-39. doi: 10.1016/j.jdermsci.2016.06.013. Epub 2016 Jul 1.  
Citation 7:
Li Chen and David R. Brigstock. Integrins and heparan sulfate proteoglycans on hepatic stellate cells (HSC) are novel receptors for HSC-derived exosomes. FEBS Lett. 2016 Dec;590(23):4263-4274. doi: 10.1002/1873-3468.12448. Epub 2016 Oct 23.
Citation 8:
Yan Sun, Sha Liu, Zhi Qiao, Zhi Shang, Zhijun Xia, Xiaomin Niu, Liqiang Qian, Yan Zhang, Liuyin Fan, Cheng-Xi Cao, Hua Xiao. Systematic comparison of exosomal proteomes from human saliva and serum for the detection of lung cancer. Anal Chim Acta. 2017 Aug 22;982:84-95. doi: 10.1016/j.aca.2017.06.005. Epub 2017 Jun 17.

FAQ

Components

Components Amount Storage
Cat.#: P101S Cat.#: P101   Cat.#: P101L
Solution A 0.6 ml 3 ml 12 ml Room temperature
Solution B 0.6 ml 3 ml 12 ml Room temperature
Solution C 0.6 ml 3 ml 12 ml Room temperature
Sample Buffer 4 ml 20 ml 80 ml Room temperature
PureExo®Column 2 10 40 Room temperature

Remarks

Because the exosome yield of this exosome isolation kit is 10-fold higher than all other kits, you can start with as little as 100 µl serum. One PureExo® Column can be used only for one reaction. Do not process more than 500 µL serum/plasma per each reaction. Otherwise it may cause column clogging.

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