Also available at    &     VWR

PureExo® Exosome Isolation kit (for cell culture media)

Why our exosome so pure?
Answer: By targeted filtration, our filter of specific binding treatment removes protein contaminant to isolate exosome at 95% high purity.        Click to see pure exosome images

Name

PureExo® Exosome Isolation kit (for cell culture media)

Cat. #

P100S
$  109.00
(  2 reactions)    order from  Fisher                               (Fisher Cat.# NC0929477)

P100
$  299.00
(10 reactions)    order from  Fisher                               (Fisher Cat.# NC1286079)

P100L
$1149.00
(40 reactions)    contact Fisher

P100K
$  269.00
(Solution A, B and C, 10 rxn)

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Exosome vs endosome

Application

Isolate pure exosome from cell culture media (supernatant). This kit yields much higher purity than exosome precipitation method. This product is for research use only.

Yield

Each reaction can process 2 ~ 4 ml cell culture medium. The yield of each reaction can yield pure exosome suspended in 50 ~ 200 µl PBS (from which 150 ~ 400 µg exosomal protein or 50 ~ 200 ng exosomal RNA can be extracted using our Exosomal RNA and Protein Extraction Kit, Cat.#: P200).

Description

P100 kit is for exosome purification / isolation at high yield and purity from cell culture media.

  • Easy to use: No ultra-centrifugation (< 2 hours)
  • 10 fold higher yield (vs. other kits and ultracentrifuge)
  • Save cost (vs. antibodies-beads method)
  • Isolate Pure exosome (>95% purity vs. 25% purity by exosome precipitation method)
  • Intact exosome (good morphology)

Use as little as 2 ml cell media to achieve high yield of exosomes for any downstream applications: EM study, exosome label, exosome subpopulation, qRT-PCR profiling of exosomal miRNAs, and gel analysis of exosomal proteins.

Shipping / Storage

Ship and store at room temperature. Upon receiving keep all bottles upright, in cool and dark place.

Shelf Life

12 months

Manual (protocol)

101Bio.com     Exosome Isolation Kit

Troubleshooting

Data Sheet

Citation

Citation 1:
Osteikoetxea X, Németh A, Sódar BW, Vukman KV, Buzás EI. Extracellular vesicles in cardiovascular diseases, are they Jedi or Sith?
J Physiol. 2016 Feb 13. doi: 10.1113/JP271336   
Citation 2:
Korbelik M, Banáth J, Zhang W, Wong F, Bielawski J, Separovic D. Ceramide and Sphingosine-1-Phosphate/Sphingosine act as Photodynamic Therapy-Elicited Damage-Associated Molecular Patterns: Release from Cells and Impact on Tumor-Associated Macrophages. J Anal Bioanal Tech. S1:009. doi: 10.4172/2155-9872.S1-009 (2014)  
Citation 3:
Salama A, Fichou N, Allard M, Dubreil L, De Beaurepaire L, Viel A, Jégou D, Bösch S, Bach JM. MicroRNA-29b modulates innate and antigen-specific immune responses in mouse models of autoimmunity. PLoS One. 2014 Sep 9;9(9):e106153. doi: 10.1371/journal.pone.0106153.  (Sold through our European distributor - Gentaur.)
Citation 4:
Marędziak M, Marycz K, Lewandowski D, Siudzińska A, Śmieszek A. Static magnetic field enhances synthesis and secretion of membrane-derived microvesicles (MVs) rich in VEGF and BMP-2 in equine adipose-derived stromal cells (EqASCs)-a new approach in veterinary regenerative medicine. In Vitro Cell Dev Biol Anim. 2015 Mar;51(3):230-40. doi: 10.1007/s11626-014-9828-0.  
Citation 5:
Tomoyo Kawakubo-Yasukochi, Masahiko Morioka, Yoshikazu Hayashi, Takuya Nishinakagawa, Mai Hazekawa, Shintaro Kawano, Seiji Nakamura, Manabu Nakashima    The SQUU-B cell line spreads its metastatic properties to nonmetastatic clone SQUU-A from the same patient through exosomes. J of Oral Biosciences   2015/09
PureExo® citation from Japan distributor Cosmo indicates interesting exosome function: "exosome derived from highly metastatic SQUU-B cells conferred metastatic ability to nonmetastatic SQUU-A cells."
Citation 6:
William G. Whitford    Exosomes: A Major Phenomenon Flying Under the Radar cellculturedish.com   2015/09
Citation 7:
Bonafede R, Scambi I, Peroni D, Potrich V, Boschi F, Benati D, Bonetti B, Mariotti R.    Exosome derived from murine adipose-derived stromal cells: Neuroprotective effect on in vitro model of amyotrophic lateral sclerosis. Exp Cell Res.   2016 Jan 1;340(1):150-8. doi: 10.1016/j.yexcr.2015.12.009. Epub 2015 Dec 18.
Citation 8:
Zhou Y, Fang L, Yu Y, Niu J, Jiang L, Cao H, Sun Q, Zen K, Dai C, Yang J.    Erythropoietin protects the tubular basement membrane by promoting the bone marrow to release extracellular vesicles containing tPA-targeting miR-144. Am J Physiol Renal Physiol.   2016 Jan 1;310(1):F27-40. doi: 10.1152/ajprenal.00303.2015. Epub 2015 Oct 14.
Citation 9:
Campbell CR, Berman AE, Weintraub NL, Tang YL.    Electrical stimulation to optimize cardioprotective exosomes from cardiac stem cells. Med Hypotheses.   2016 Mar;88:6-9. doi: 10.1016/j.mehy.2015.12.022. Epub 2016 Jan 11.
Citation 10:
Zhu D, Liu Z, Wang F. Effect of exosome extracted from 3T3 on proliferation of mouse colorectal cancer cells CT26. Chin J of Exp Surg. 2015 July;32(7):1634-36
Citation 11:
Rager T, Olson J, Zhou Y, Wang Y, Besner G. Exosomes Secreted from Bone Marrow-Derived Mesenchymal Stem Cells Protect the Intestines from Experimental Necrotizing Enterocolitis. J Pediatr Surg. 2016 March;S0022-3468(16)00149-4
Citation 12:
Li X, Liu L, Yang J, Yu Y, Chai J, Wang L, Ma L, Yin H. Exosome Derived From Human Umbilical Cord Mesenchymal Stem Cell Mediates MiR-181c Attenuating Burn-induced Excessive Inflammation. EBioMedicine. 2016, http://dx.doi.org/10.1016/j.ebiom.2016.04.030
Citation 13:
Masahiko Moriokaa, Tomoyo Kawakubo-Yasukochia, Yoshikazu Hayashi, Mai Hazekawa, Takuya Nishinakagawa, Kazuhiko Ono, Shintaro Kawano, Seiji Nakamura, Manabu Nakashima. Exosomes from oral squamous carcinoma cell lines, SQUU-A and SQUU-B, define the tropism of lymphatic dissemination. J of Oral Biosciences. 2016, http://dx.doi.org/10.1016/j.job.2016.05.001i
Citation 14:
Kiyoshi Takahara, Masaaki Ii, Teruo Inamoto, Takatoshi Nakagawa, Naokazu Ibuki, Yuki Yoshikawa, Takuya Tsujino, Taizo Uchimoto, Kenkichi Saito, Tomoaki Takai, Naoki Tanda, Koichiro Minami, Hirofumi Uehara, Kazumasa Komura, Hajime Hirano, Hayahito Nomi, Satoshi Kiyama, Michio Asahi, and Haruhito Azuma. microRNA-145 Mediates the Inhibitory Effect of Adipose Tissue-Derived Stromal Cells on Prostate Cancer. Stem Cells Dev. 2016 Sep 1;25(17):1290-8. doi: 10.1089/scd.2016.0093. Epub 2016 Aug 18.
Citation 15:
Alice Busato, Roberta Bonafede, Pietro Bontempi, Ilaria Scambi, Lorenzo Schiaffino, Donatella Benati, Manuela Malatesta, Andrea Sbarbati, Pasquina Marzola, and Raffaella Mariotti. Magnetic resonance imaging of ultrasmall superparamagnetic iron oxide-labeled exosomes from stem cells: a new method to obtain labeled exosomes. Int J Nanomedicine. 2016; 11: 2481–2490.
Citation 16:
Li Chen and David R. Brigstock. Integrins and heparan sulfate proteoglycans on hepatic stellate cells (HSC) are novel receptors for HSC-derived exosomes. FEBS Lett. 2016 Dec;590(23):4263-4274. doi: 10.1002/1873-3468.12448. Epub 2016 Oct 23.
Citation 17:
Donald G. Phinney and Mark F. Pittenger Concise Review: MSC-Derived Exosomes for Cell-Free Therapy. Stem Cells. 2017 Apr;35(4):851-858. doi: 10.1002/stem.2575. Epub 2017 Mar 10.

FAQ

Components

Components Amount Storage
Cat.#: P100S Cat.#: P100   Cat.#: P100L
Solution A (blue) 0.5 ml 2.5 ml 10 ml Room temperature
Solution B 0.5 ml 2.5 ml 10 ml Room temperature
Solution C 2 ml 10 ml 10 ml x 4 Room temperature
PureExo® Column 2 10 40 Room temperature

Remarks

Because the exosome yield of this exosome isolation kit is 10 fold higher than all other kits, you can start with only 2 mL medium. The maximum medium volume of each reaction is 4 ml from at most 5 x 105 cells. One PureExo® Column is only for one reaction. Do not exceed 4 mL medium or 5 x 105 cells to avoid indistinct layer separation and column clogging.

Our kits optimize your exosome research result

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Good size distribution of exosomes isolated by our PureExo® kit
   (See larger screen click: )

Nanoparticle tracking analysis shows good size distribution, morphology and amount of exosome isolated by 101bio PureExo® kit.

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